Expression and function analysis of Rac1 homolog in Chinese shrimp Fenneropenaeus chinensis
Expression and function analysis of Rac1 homolog in Chinese shrimp Fenneropenaeus chinensis
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Rac1同源物在中国明对虾中的表达及功能分析
DOI：
10.1016/j.fsi.2013.07.006
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发表时间：
2013-09-01
影响因子：
4.7
通讯作者：
Li, Shihao
中科院分区：
文献类型：
--
作者：
Chi, Yanhong;Li, Fuhua;Li, Shihao
Rac1是一种普遍存在的GTP结合蛋白，在多种细胞过程中起着至关重要的作用。本研究从中国明对虾（Fenneropenaeus chinensis）中克隆了一个Rac 1同源基因（FcRac 1）。FcRac 1的开放阅读框（ORF）由579 bp组成，编码192个氨基酸。FcRac 1氨基酸序列的预测分子量（MW）为21.46 kDa，理论pI为8.62。同源性分析表明，Rac1的氨基酸序列在不同物种间具有较高的保守性。系统进化分析表明，FcRac 1与其他节肢动物的Rac 1蛋白有较近的亲缘关系。FcRac 1在血细胞中的表达水平最高。原位杂交检测表明，它分布在所有类型的血细胞。重组蛋白FcRac 1具有明显的GT3活性。FcRac 1基因在对虾幼虾体内的转录水平在细菌和WSSV攻击后发生了变化。本研究结果提示FcRac 1在对虾天然免疫中可能起重要作用。(C)2013爱思唯尔有限公司保留所有权利。
Rac1 is a ubiquitous GTP-binding protein that plays a crucial role in multiple cellular processes. In the present study, a Rac1 homolog (FcRac1) was cloned from the Chinese shrimp Fenneropenaeus chinensis. The open reading frame (ORF) of FcRac1 consists of 579 bp encoding 192 aa. The predicted molecular weight (MW) of the deduced amino acid sequence of FcRac1 was 21.46 kDa, and its theoretical pI was 8.62. Homology analysis showed that the amino acid sequence of Rac1 had high conservation among those from different species. Phylogenetic analysis showed that FcRac1 closely related to Rac1 proteins from other arthropods. FcRac1 showed the highest expression level in the hemocytes. In situ hybridization detection showed that it distributed in all types of hemocytes. Recombinant protein of FcRac1 showed apparent activity of GTPase. The transcription of FcRac1 in juvenile shrimp changed after bacteria or WSSV challenge. The present data suggests that FcRac1 might play important roles in the innate immunity of shrimp. (C) 2013 Elsevier Ltd. All rights reserved.