Betaine improves the PCR amplification of GC-rich DNA sequences
Betaine improves the PCR amplification of GC-rich DNA sequences
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DOI：
10.1093/nar/25.19.3957
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发表时间：
1997-10-01
影响因子：
14.9
通讯作者：
Loening, SA
中科院分区：
文献类型：
--
作者：
Henke, W;Herdel, K;Loening, SA
甜菜碱促进了前列腺特异性膜抗原mRNA的两个可变剪接变体的共扩增以及c-jun的编码cDNA区域的扩增，这表明甜菜碱通过减少由富含GC的区域引起的二级结构的形成来改善这些基因的扩增，因此，可能普遍适用于改善富含GC的DNA序列的扩增。
Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.