Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities
Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities
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DOI：
10.1128/aem.72.4.3016-3020.2006
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发表时间：
2006-04-01
影响因子：
4.4
通讯作者：
Hoegh-Guldberg, O
中科院分区：
文献类型：
--
作者：
Ainsworth, TD;Fine, M;Hoegh-Guldberg, O
微生物群落在珊瑚礁群落的功能中发挥着重要作用。然而，珊瑚组织和内共生体广泛的自发荧光限制了标准荧光原位杂交（FISH）技术用于鉴定珊瑚相关细菌群落的应用。本研究通过将FISH和光谱成像相结合来克服这些局限性。
Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. This study overcomes these limitations by combining FISH and spectral imaging.