Differentiation of the avian latissimus dorsi primordium: Analysis of fiber type expression using the myosin ATPase histochemical reaction
Differentiation of the avian latissimus dorsi primordium: Analysis of fiber type expression using the myosin ATPase histochemical reaction
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禽类背阔肌原基的分化：使用肌球蛋白 ATP 酶组织化学反应分析纤维类型表达
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
E. Cosmos
中科院分区：
文献类型：
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作者：
J. Butler;E. Cosmos
通常情况下，快速收缩纤维的成年禽后背阔肌（PLD）肌肉表达碱稳定的肌球蛋白ATP酶组织化学活性，而慢紧张纤维的前背阔肌（ALD）肌肉表现出双重的酸和碱的稳定性。由于PLD纤维交叉神经支配的ALD肌肉的神经也表现出双重的稳定性，我们试图确定ALD神经是否诱导这种新的表型从头或去抑制胚胎表型抑制在进一步发展过程中在卵。鸡胚从第2天开始在卵内冷冻，并确定肌球蛋白ATP酶组织化学谱的发展碱和酸预孵育后的肌源性细胞注定形成ALD和PLD肌肉。此外，银胆碱酯酶反应的连续切片，以探讨早期肌肉神经的相互作用在卵。我们的研究结果表明，所有肌原细胞的主要背肌质量，包括那些未来的ALD和PLD肌肉，表现出他们的第一个肌球蛋白ATP酶反应阶段（ST）25。此时（卵内4.5天），仅表达碱稳定的酶活性。到St 28（5.5天），一个明显的背阔肌（LD）原基已经从背侧肌肉块中分裂出来，整个原基仍然只表现出碱稳定性。随着发育的进行，LD原基分离成可识别的前部和后部区域（St 29，6.0天至St 30，6.5天），未来的PLD肌肉继续仅表达碱稳定性，而未来的ALD肌肉现在表现出双重稳定性。到St 32（7.5天），这两块肌肉完全分离，在卵内发育的剩余时间内，成人慢强直与快颤肌纤维的特征性轮廓保持不变。因此，我们的研究结果表明，在交叉再支配卵外，ALD神经诱导表达的一个新的表型从来没有表现出潜在的快速收缩PLD纤维在整个胚胎期，包括其初期形成。此外，我们的研究结果表明，肌球蛋白ATP酶活性的组织化学反应提供了一个很好的酶标记，既为肌原细胞和单个肌肉原基的识别，并在卵内发育的第一周检测肌纤维类型分化。
Typically, fast twitch fibers of the adult avian posterior latissimus dorsi (PLD) muscle express alkali stable myosin ATPase histochemical activity, whereas slow tonic fibers of the anterior latissimus dorsi (ALD) muscle exhibit dual acid and alkali stability. Since PLD fibers cross-reinnervated by the nerve of the ALD muscle also demonstrate dual stability, we sought to determine whether the ALD nerve induces this new phenotype de novo or de-represses an embryonic phenotype repressed during further development in ovo. Chick embryos from Day 2 in ovo onward were frozen in toto, and the development of myosin ATPase histochemical profiles following alkali and acid preincubation was determined for myo-genic cells destined to form the ALD and PLD muscles. In addition, the silver cholinesterase reaction was employed on serial sections to investigate early muscle-nerve interaction in ovo. Our results indicate that all myogenic cells of the primary dorsal muscle mass, including those of the future ALD and PLD muscles, demonstrate their first myosin ATPase response at Stage (St) 25. At this time (4.5 days in ovo), only alkali stable enzymic activity is expressed. By St 28 (5.5 days) a distinct latissimus dorsi (LD) primordium has cleaved from the dorsal muscle mass and the entire primordium still exhibits only alkali stability. As development proceeds and the LD primordium separates into recognizable anterior and posterior regions (St 29, 6.0 days through St 30, 6.5 days), the future PLD muscle continues to express only alkali stability, whereas the future ALD muscle now demonstrates dual stability. By St 32 (7.5 days), these two muscles are completely separated, and during the remainder of in ovo development the profiles characteristic of adult slow tonic versus fast twitch fibers remain unaltered. Thus, our results indicate that, during cross-reinnervation ex ovo, the ALD nerve induces the expression of a new phenotype never exhibited by potential fast twitch PLD fibers during the entire embryonic period, including their incipient formation. Moreover, our results indicate that the histochemical reaction for myosin ATPase activity provides an excellent enzymic marker both for the identification of myogenic cells and individual muscle primordia, and, for the detection of muscle fiber type differentiation during the first week of development in ovo.