Larval diet affects adult reproduction but not survival regardless of injury and infection stress in Drosophila melanogaster
Larval diet affects adult reproduction but not survival regardless of injury and infection stress in Drosophila melanogaster
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DOI：
10.1101/2020.10.16.342618
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发表时间：
2020-10
期刊：
影响因子：
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通讯作者：
E. Savola;P. Vale;C. Walling
中科院分区：
文献类型：
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作者：
E. Savola;P. Vale;C. Walling
早期生活条件对许多生活史特征有着深远的影响。特别是，早期的饮食影响幼年的发育，成年的生存和繁殖。早期的饮食也会影响成年人承受饥饿、温度和干燥等压力的能力。然而，人们不太清楚早期饮食如何影响成年人对感染的反应能力。在这里，我们测试是否不同的雌性果蝇幼虫的饮食（通过改变蛋白质与碳水化合物的比例，P：C）影响长期的反应，伤害和感染的细菌病原体嗜虫假单胞菌。考虑到以前的工作操纵成人饮食P：C，我们预测，从幼虫的成人更有可能生存感染和增加繁殖，但寿命较短，衰老速度加快。对于幼虫发育，我们预测低P：C将导致较长的发育时间和较低的生存力。我们发现，早期的生活和一生的鸡蛋产量最高的中间到高幼虫P：C饮食，但没有影响幼虫P：C对成人的生存。幼虫饮食对感染后的存活或繁殖没有影响。幼虫在中等P：C下发育最快，卵至蛹和卵至成虫的存活率在较高P：C下较高。总体而言，尽管幼虫P：C影响本研究中测量的几个性状，但我们没有看到任何证据表明幼虫P：C改变了感染或损伤对成虫存活和早期生活以及终生繁殖的影响。综上所述，这些数据表明，幼虫的饮食似乎有一个有限的影响成人感染的反应。
Early-life conditions have profound effects on many life-history traits. In particular, early-life diet affects both juvenile development, and adult survival and reproduction. Early-life diet also has consequences for the ability of adults to withstand stressors such as starvation, temperature and desiccation. However, it is less well known how early-life diet influences the ability of adults to respond to infection. Here we test whether varying the larval diet of female Drosophila melanogaster (through altering protein to carbohydrate ratio, P:C) influences the long-term response to injury and infection with the bacterial pathogen Pseudomonas entomophila. Given previous work manipulating adult dietary P:C, we predicted that adults from larvae raised on higher P:C diets would be more likely to survive infection and have increased reproduction, but shorter lifespans and an increased rate of ageing. For larval development, we predicted that low P:C would lead to a longer development time and lower viability. We found that early-life and lifetime egg production were highest at intermediate to high larval P:C diets, but there was no effect of larval P:C on adult survival. Larval diet had no effect on survival or reproduction post-infection. Larval development was quickest on intermediate P:C and egg-to-pupae and egg-to-adult viability were higher on higher P:C. Overall, despite larval P:C affecting several traits measured in this study, we saw no evidence that larval P:C altered the consequence of infection or injury for adult survival and early-life and lifetime reproduction. Taken together, these data suggest that larval diets appear to have a limited impact on adult response to infection.