Glycerol and nonesterified fatty acid metabolism in human muscle and adipose tissue in vivo
Glycerol and nonesterified fatty acid metabolism in human muscle and adipose tissue in vivo
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DOI：
10.1152/ajpendo.1999.276.2.e233
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发表时间：
1999-02-01
影响因子：
5.1
通讯作者：
Miles, JM
中科院分区：
文献类型：
--
作者：
Coppack, SW;Persson, M;Miles, JM
为了确定甘油和非酯化脂肪酸（NEFA）从脂肪组织释放之间的关系，并测试前臂肌肉和腹部脂肪组织是否能够从循环中提取这两种脂解产物，对13名男性受试者在联合输注放射性标记的棕榈酸酯和甘油期间禁食过夜后进行了研究。在输注葡萄糖之前和输注2小时期间，从桡动脉、前臂深静脉和腹部浅静脉采集血液样本，输注时间与7 mg.kg相似。总NEFA与甘油的出现率之比在基线期间与3/1相似，但在葡萄糖输注期间降至1.3/1。有显着提取甘油和NEFA的前臂肌肉。相比之下，脂肪组织没有明显的甘油摄取。脂肪组织NEFA摄取在基线期检测不到，但在葡萄糖输注期间变得显著。这些数据表明，在禁食过夜后，脂肪组织中NEFA的原位再酯化非常少或没有。在葡萄糖输注过程中，脂蛋白脂肪酶作用产生的甘油分数明显增加，原位再酯化作用增加。
To determine the relationship between glycerol and nonesterified fatty acid (NEFA) release from adipose tissue, and to test whether forearm muscle and abdominal adipose tissue are capable of extracting these two lipolytic products from the circulation, 13 male subjects were studied after an overnight fast during combined infusion of radiolabeled palmitate and glycerol. Blood samples were taken from a radial artery, a deep forearm vein, and a superficial abdominal vein before and during a 2-h infusion of glucose at similar to 7 mg.kg(-1).min(-1) The ratio of the appearance rates of total NEFA to glycerol was similar to 3/1 during the baseline period but decreased to 1.3/1 during glucose infusion. There was significant extraction of both glycerol and NEFA by forearm muscle. In contrast, there was no apparent uptake of glycerol by adipose tissue. Adipose tissue NEFA uptake was undetectable during the baseline period but became significant during glucose infusion. These data indicate that there is very little to no in situ reesterification of NEFA in adipose tissue after an overnight fast. During glucose infusion, there was apparently a relative increase in the fraction of glycerol derived from the action of lipoprotein lipase and an increase in reesterification in situ.