In Vitro Release of Adenosine Triphosphate from the Urothelium of Human Bladders with Detrusor Overactivity, Both Neurogenic and Idiopathic
In Vitro Release of Adenosine Triphosphate from the Urothelium of Human Bladders with Detrusor Overactivity, Both Neurogenic and Idiopathic
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DOI：
10.1016/j.eururo.2009.11.042
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发表时间：
2010-06-01
期刊：
影响因子：
23.4
通讯作者：
Chess-Williams, Russell
中科院分区：
文献类型：
--
作者：
Kumar, Vivek;Chapple, Christopher R.;Chess-Williams, Russell
背景：越来越多的证据表明非肾上腺素能-非胆碱能神经传递在膀胱功能障碍的发病机制中发挥作用。目的：在这组实验中，我们通过量化特发性逼尿肌过度活动（IDO）和神经源性逼尿肌过度活动患者尿路上皮释放的三磷酸腺苷（ATP）量，评估了尿路上皮对嘌呤能活性的贡献。 (NDO) 并将这些释放与对照的释放进行比较。设计、设置和参与者：本研究包括具有尿动力学和临床证明的 NDO (n = 8) 和 IDO (n = 8) 的膀胱组织。通过机械拉伸以及电刺激和 ATP 来刺激仔细解剖的尿路上皮；测量：我们使用 Lucy Anthos 1 光度计（Anthos Labtec Instruments GmBH，Wals，奥地利）进行测定。使用 Stingray 软件（Dazdaq Ltd，布莱顿，英国）对结果进行分析。结果和局限性：与对照组相比，机械拉伸和电场刺激 (EFS) 都会导致两组过度活动的组织中 ATP 释放增加； IDO 尿路上皮 (2416.7 +/- 479.8 pmol/g [p < 0.005]) 的这种上升甚至比 NDO 尿路上皮 (133.1 +/- 22.4 pmol/g [p < 0.01]) 更显着；对照的值为 77.6 +/- 16.2 pmol/g。与 IDO 以及对照组相比，NDO 膀胱中机械拉伸后的 ATP 释放对河豚毒素更敏感，ATP 水平从 233.5 +/- 20.7 pmol/g 下降至 107.2 +/- 11.6 pmol/g，以基础水平的百分比表示（p < 0.002）。实验在体外进行，女性患者处于围绝经期和绝经后状态。结论：这些实验表明，与对照组相比，接受 NDO 和 IDO 的膀胱尿路上皮 ATP 释放量显着增加。 NDO 从膀胱释放的大部分 ATP 主要来自神经元。 (c) 2009 年欧洲泌尿外科协会。由 Elsevier B.V. 出版。保留所有权利。
Background: There is increased evidence to suggest a role for nonadrenergic-noncholinergic neurotransmission in the pathogenesis of bladder dysfunction.Objective: In this set of experiments, we have assessed the contribution of the urothelium to purinergic activity by quantifying the amount of adenosine triphosphate (ATP) released from the urothelium of patients with idiopathic detrusor overactivity (IDO) and with neurogenic detrusor overactivity (NDO) and comparing these releases to those of controls.Design, setting, and participants: Bladder tissue with urodynamically and clinically proven NDO (n = 8) and IDO (n = 8) were included in this study. The carefully dissected urothelium was stimulated by mechanically stretching as well as electrically stimulating and the ATP; thus, release was quantified.Measurements: We used a Lucy Anthos 1 luminometre (Anthos Labtec Instruments GmBH, Wals, Austria) to perform the assay. The results were analysed using Stingray software (Dazdaq Ltd, Brighton, UK).Results and limitations: Both mechanical stretch and electric field stimulation (EFS) led to increased ATP release in both sets of tissues with overactivity compared to the controls; this rise was even more significant for the IDO urothelium (2416.7 +/- 479.8 pmol/g [p < 0.005]) than for the NDO urothelium (133.1 +/- 22.4 pmol/g [p < 0.01]); values for the controls were 77.6 +/- 16.2 pmol/g. ATP release following mechanical stretch was more sensitive to tetrodotoxin in bladders with NDO compared to those with IDO as well as to the controls, with ATP levels falling from 233.5 +/- 20.7 pmol/g to 107.2 +/- 11.6 pmol/g, expressed as percentage of basal levels (p < 0.002). The experiments were performed in vitro, and the female patientswere a mix of peri- and postmenopausal states.Conclusions: These experiments suggested a significant rise in ATP release from the urothelium of bladders with NDO as well as those with IDO in comparison to controls. Most of the ATP released from bladders with NDO is primarily from neuronal sources. (c) 2009 European Association of Urology. Published by Elsevier B.V. All rights reserved.