Crystallization and preliminary structural analysis of dibenzothiophene monooxygenase (DszC) from Rhodococcus erythropolis
Crystallization and preliminary structural analysis of dibenzothiophene monooxygenase (DszC) from Rhodococcus erythropolis
复制标题
红平红球菌二苯并噻吩单加氧酶 (DszC) 的结晶和初步结构分析。
DOI：
10.1107/s1744309113011172
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发表时间：
2013-06-01
影响因子：
0.9
通讯作者：
Li, Xin
中科院分区：
文献类型：
--
作者：
Duan, Xiaolu;Zhang, Liang;Li, Xin
二苯并噻吩（DBT）及其衍生物是化石燃料中常见的典型硫化物。这些化合物显示出对工业中常用的加氢脱硫处理的抗性。二苯并噻吩单加氧酶（DszC）负责DBT的氧化，这是DBT酶促脱硫4S途径中的第一步和限速步骤。本文报道了红平红球菌DS-3中DszC的晶体结构。晶体属P1空间群，晶胞参数a = 96.16，B = 96.27，c = 98.56 °，α = 81.03，β = 67.57，γ = 85.84°。为了确定相，从SeMet衍生物DszC晶体收集SAD X射线衍射数据，该晶体也属于空间群P1，具有晶胞参数a = 95.379，B = 95.167，c = 94.891 °，α = 87.046，β = 70.536，γ = 79.738°。DszC的进一步结构分析正在进行中。
Dibenzothiophene (DBT) and its derivatives are typical sulfur compounds found in fossil fuels. These compounds show resistance to the hydrodesulfurization treatment that is commonly used in industry. Dibenzothiophene monooxygenase (DszC) is responsible for the oxidation of DBT, which is the first and the rate-limiting step in the DBT enzymatic desulfurization 4S pathway. In this study, the crystal structure of DszC from Rhodococcus erythropolis DS-3 is reported. The crystal of native DszC belonged to space group P1, with unit-cell parameters a = 96.16, b = 96.27, c = 98.56 Å, α = 81.03, β = 67.57, γ = 85.84°. To determine the phase, SAD X-ray diffraction data were collected from a SeMet-derivative DszC crystal, which also belonged to space group P1, with unit-cell parameters a = 95.379, b = 95.167, c = 94.891 Å, α = 87.046, β = 70.536, γ = 79.738°. Further structural analysis of DszC is in progress.