Clinical significance of overexpressed cyclin-dependent kinase subunits 1 and 2 in esophageal carcinoma.
Clinical significance of overexpressed cyclin-dependent kinase subunits 1 and 2 in esophageal carcinoma.
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食管癌中过度表达的细胞周期蛋白依赖性激酶亚基 1 和 2 的临床意义。
DOI：
10.1111/dote.12013
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发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
Zhang ZY
中科院分区：
文献类型：
--
作者：
Wang JJ;Fang ZX;Ye HM;You P;Cai MJ;Duan HB;Wang F;Zhang ZY
哺乳动物细胞周期蛋白依赖性激酶亚基 (Cks) 家族有两个成员：Cks1 和 Cks2。据报道，Cks1 和 Cks2 的过度表达与多种恶性肿瘤（包括前列腺癌和肝细胞癌）的高侵袭性和不良预后相关。然而，Cks1 和 Cks2 在食管癌中是否过度表达仍不清楚。探讨Cks家族的过度表达在食管癌中是否具有临床相关性，以及Cks1和Cks2的表达模式是否可以作为食管癌的生物标志物。采用实时定量逆转录聚合酶链反应、免疫组化和Western blot分析分别检测Cks1和Cks2在mRNA和蛋白水平的表达。分析食管癌中Cks1或Cks2表达与临床特征以及p27(kip1)表达的关系。与癌旁癌组织相比，食管癌中Cks1的mRNA水平和蛋白水平的表达分别为58%和54%，Cks2的mRNA水平和蛋白水平分别为65%和61%。肿瘤组织中Cks1和Cks2的表达均与p27(kip1)蛋白水平呈负相关。此外，Cks1和Cks2在食管癌中的过度表达与食管癌不良的病理特征密切相关，包括较高的肿瘤组织学分级、区域淋巴结侵犯和肿瘤栓塞。 Cks1和Cks2的过度表达与食管癌的侵袭性肿瘤行为相关。需要进一步努力确定 Cks1 和 Cks2 的过度表达是否可以作为食管癌的新型生物标志物。
The mammalian cyclin-dependent kinase subunit (Cks) family has two members, Cks1 and Cks2. Overexpression of Cks1 and Cks2 has been reported to be associated with high aggressiveness and poor prognosis in several malignancies, including prostate and hepatocellular carcinomas. However, whether Cks1 and Cks2 are overexpressed in esophageal carcinoma remains uncharacterized. To investigate whether overexpression of the Cks family is clinically relevant in esophageal carcinoma, and whether expression patterns of Cks1 and Cks2 can serve as biomarkers for esophageal carcinoma. Real-time quantitative reverse transcription polymerase chain reaction, immunohistochemistry, and Western blot analyses were applied to detect the expression of Cks1 and Cks2 at the mRNA and protein levels, respectively. The associations between Cks1 or Cks2 expressions and clinical features and p27(kip1) expressions in esophageal carcinoma were analyzed. Comparing with the adjacent noncancerous tissues, esophageal carcinoma exhibited elevated expression of Cks1 in 58% cases at the mRNA level and 54% cases at the protein level, and elevated expression of Cks2 in 65% cases at the mRNA level and 61% cases at the protein level, respectively. The expressions of both Cks1 and Cks2 were negatively associated with the p27(kip1) protein level in the tumor tissues. Furthermore, overexpression of Cks1 and Cks2 in esophageal carcinoma was closely associated with poor pathological features of esophageal carcinoma, including higher histologic grade of tumor, regional lymph nodes invasion, and neoplastic embolus. Overexpression of Cks1 and Cks2 is associated with the aggressive tumor behaviors of esophageal carcinoma. Further efforts are needed to determine whether overexpression of Cks1 and Cks2 can serve as novel biomarkers for esophageal carcinoma.