Molecular features of non-B, non-C hepatocellular carcinoma: a PCR-array gene expression profiling study
Molecular features of non-B, non-C hepatocellular carcinoma: a PCR-array gene expression profiling study
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DOI：
10.1016/s0168-8278(03)00473-2
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发表时间：
2003-12-01
影响因子：
25.7
通讯作者：
Monden, M
中科院分区：
文献类型：
--
作者：
Kurokawa, Y;Matoba, R;Monden, M
背景/目的：肝细胞癌 (HCC) 通常在丙型肝炎或乙型肝炎病毒引起的慢性肝脏炎症后发生。通过对病因不明的罕见类型 HCC 进行表达谱分析，我们试图找到在没有病毒影响的情况下负责肝癌发生每个步骤的关键基因。方法：我们使用 68 个非 B、非 C 肝组织（20 个 HCC、17 个非肿瘤、31 个正常肝脏）进行表达谱分析，PCR 阵列携带已知在肝组织中表达的 3072 个基因。为了选择差异表达的基因，我们进行了随机排列测试。使用加权投票分类算法来确认基因选择的可靠性。然后我们将这些基因与之前的表达谱研究的结果进行比较。结果：通过随机排列测试总共选出了220个差异表达基因。通过留一交叉验证、额外的 PCR 阵列数据集和斯坦福大学 DNA 微阵列数据集，使用这些基因的分类准确度分别为 91.8%、92.0% 和 100.0%。通过将我们的结果与之前关于病毒感染的 HCC 的报道进行比较，在一些研究中普遍选择了四个基因（ALB、A2M、ECHS1 和 IGFBP3）。 结论：通过 PCR 阵列选择的 220 个差异表达基因可能在没有病毒影响的情况下导致肝癌的发生。 (C) 2003 年欧洲肝脏研究协会。由 Elsevier B.V. 出版。保留所有权利。
Background/Aims: Hepatocellular carcinoma (HCC) usually develops following chronic liver inflammation caused by hepatitis C or B virus. Through expression profiling in a rare type of HCC, for which the causes are unknown, we sought to find key genes responsible for each step of hepatocarcinogenesis in the absence of viral influence.Methods: We used 68 non-B, non-C liver tissues (20 HCC, 17 non-tumor, 31 normal liver) for expression profiling with PCR-array carrying 3072 genes known to be expressed in liver tissues. To select the differentially expressed genes, we performed random permutation testing. A weighted voting classification algorithm was used to confirm the reliability of gene selection. We then compared these genes with the results of previous expression profiling studies.Results: A total of 220 differentially expressed genes were selected by random permutation tests. The classification accuracies using these genes were 91.8, 92.0 and 100.0% by a leave-one-out cross-validation, an additional PCR-array dataset and a Stanford DNA microarray dataset, respectively. By comparing our results with previous reports on virus-infected HCC, four genes (ALB, A2M, ECHS1 and IGFBP3) were commonly selected in some studies.Conclusions: The 220 differentially expressed genes selected by PCR-array are potentially responsible for hepatocarcinogenesis in the absence of viral influence. (C) 2003 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.