Cell Therapy Based on Adipose Tissue-Derived Stromal Cells Promotes Physiological and Pathological Wound Healing
Cell Therapy Based on Adipose Tissue-Derived Stromal Cells Promotes Physiological and Pathological Wound Healing
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DOI：
10.1161/atvbaha.108.178962
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发表时间：
2009-04-01
影响因子：
8.7
通讯作者：
Tamarat, R.
中科院分区：
文献类型：
--
作者：
Ebrahimian, T. G.;Pouzoulet, F.;Tamarat, R.
目的：我们假设脂肪组织可能含有具有皮肤和血管生成潜能的祖细胞。方法与结果：将脂肪组织来源的间质细胞（ADSCs）给予未辐照和局部辐照小鼠皮肤穿孔创面（20 Gy）。在第14天，ADSCs促进了照射和未照射小鼠皮肤伤口愈合，增强了伤口闭合、粘弹性和胶原组织分泌。有趣的是，gfp阳性的ADSCs在体内融入真皮和表皮组织，并表达表皮标记物K5和K14。在角质形成细胞培养基中培养的ADSCs分化为K5和k14阳性细胞，并产生高水平的KGF。在第7天，通过激光多普勒成像、毛细血管密度和VEGF血浆水平评估，ADSCs还改善了照射和未照射动物的皮肤血液灌注。gfp阳性的ADSCs在体内并入毛细血管结构并表达内皮细胞标志物CD31。最后，原位间期荧光杂交显示，少数ADSCs具有与内源性角质形成细胞融合的潜力。结论：adscs通过促进皮肤上皮再生和血管生成参与皮肤创面的生理和病理愈合。因此，脂肪系细胞代表了治疗性皮肤伤口愈合的新细胞来源。（中华动脉血管杂志，2009;29:503-510）
Objective-We hypothesized that adipose tissue may contain progenitors cells with cutaneous and angiogenic potential.Methods and Results-Adipose tissue-derived stroma cells (ADSCs) were administrated to skin punched wounds of both nonirradiated and irradiated mice (20 Gy, locally). At day 14, ADSCs promoted dermal wound healing and enhanced wound closure, viscolesticity, and collagen tissue secretion in both irradiated and nonirradiated mice. Interestingly, GFP-positive ADSCs incorporated in dermal and epidermal tissue in vivo and expressed epidermal markers K5 and K14. Cultured ADSCs in keratinocyte medium have been shown to differentiate into K5- and K14-positive cells and produced high levels of KGF. At Day 7, ADSCs also improved skin blood perfusion assessed by laser Doppler imaging, capillary density, and VEGF plasma levels in both irradiated and nonirradiated animals. GFP-positive ADSCs incorporated into capillary structures in vivo and expressed the endothelial cell marker CD31. Finally, in situ interphase fluorescence hybridization showed that a small number of ADSCs have the potential to fuse with endogenous keratinocytes.Conclusion-ADSCs participate in dermal wound healing in physiological and pathological conditions by their ability to promote reepithelialization and angiogenesis. Hence, adipose lineage cells represent a new cell source for therapeutic dermal wound healing. (Arterioscler Thromb Vasc Biol. 2009; 29: 503-510.)