Baculovirus expression vectors : choice of expression vector.
Baculovirus expression vectors : choice of expression vector.
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DOI：
10.1385/0-89603-191-8:287
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发表时间：
1992-01-01
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影响因子：
--
通讯作者：
Emery, V C
中科院分区：
文献类型：
--
作者：
Emery, V C
杆状病毒作为有效的害虫防治剂已使用多年；然而，分子病毒学家对它们的兴趣源于它们作为独立辅助病毒表达载体的利用，用于在真核环境中生产蛋白质。导致该系统开发的开创性工作来自美国德克萨斯农工大学 Max Summers 实验室。自诞生以来，该系统已越来越多地被用来表达广泛的真核蛋白（参考文献 1 中的综述），并已进行了广泛的修改 (2)，特别是英国牛津病毒学和环境微生物学研究所的 David Bishop 和 Bob Possee。这项工作导致了单个和多个杆状病毒表达载体的构建。关于杆状病毒主题的广泛评论是可用的 (1,3-5)，包括专门用于其作为表达系统的方法手册 (6)。第 26 - 28 章旨在提供杆状病毒作为表达载体的实际说明，以及产生包含待表达基因的杆状病毒转移载体所需的实验方法、表达该基因产物的重组体的产生和选择，以及可用于处理杆状病毒产生的蛋白质的下游方法。
Baculoviruses have been used for many years as effective pest-control agents; however, their interest to molecular virologists stems from their exploitation as helper-independent viral expression vectors for the production of proteins in a eukaryotic environment. The pioneering work that led to the development of the system came from the laboratory of Max Summers at Texas A & M, USA. Since its inception, the system has been increasingly utilized to express a broad range of eukaryotic proteins (reviewed in ref. 1) and has been extensively modified (2), in particular by David Bishop and Bob Possee at the Institute of Virology and Environmental Microbiology, Oxford, UK. This work has led to the construction of single and multiple baculovirus expression vectors. Extensive reviews on the subject of baculoviruses are available (1,3-5), including a manual of methods specific for their use as expression systems (6). Chapters 26 - 28 aim to provide a practical account of baculoviruses as expression vectors, along with the experimental methodologies required to produce a baculovirus transfer vector containing the gene to be expressed, the generation and selection of a recombinant expressing this gene product, and the methods available for processing downstream the baculovirus-produced protein.