Precise Exchange of the Helper-Component Proteinase Cistron Between Soybean mosaic virus and Clover yellow vein virus: Impact on Virus Viability and Host Range Specificity
Precise Exchange of the Helper-Component Proteinase Cistron Between Soybean mosaic virus and Clover yellow vein virus: Impact on Virus Viability and Host Range Specificity
复制标题
大豆花叶病毒和三叶草黄脉病毒之间辅助成分蛋白酶顺反子的精确交换：对病毒活力和宿主范围特异性的影响
DOI：
10.1094/phyto-06-19-0193-fi
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发表时间：
2020
期刊：
影响因子：
3.2
通讯作者：
Hajimorad M. R.
中科院分区：
文献类型：
--
作者：
Wang Y.;Xu W.;Abe J.;Nakahara K. S.;Hajimorad M. R.
大豆花叶病毒和三叶草黄脉病毒是马铃薯病毒科马铃薯病毒属的两个确定种。大豆花叶病毒n （SMV-N）对栽培大豆（Glycine max）基因型和野生大豆（G. soja）具有良好的适应性，而在接种的蚕豆（Vicia faba）中检测不到。三叶草黄静脉病毒30号(ClYVV-No。30)对蚕豆和野生大豆具有全身侵染能力；然而，它只在局部感染栽培大豆基因型。在本研究中，SMV-N也会局部感染蚕豆；因此，蚕豆是SMV-N的宿主。基于这些观察结果，我们推测蚕豆缺乏SMV-N和ClYVV-No的全身感染。在栽培大豆中，30是由于多功能辅助成分蛋白酶（HC-Pro）在这些寄主中不相容。选择HC-Pro作为靶标的逻辑是基于其在系统运动中的既定功能，并且是多病毒宿主范围特异性的相关因素。为了验证这一假设，我们在SMV-N和clyv - no之间精确交换HC-Pro顺子，构建了嵌合体。30.。接种后，两种嵌合体都能在感染中存活，但重组病毒的宿主范围特异性与亲本病毒没有差异。这些观察结果表明：(i) SMV-N和clyv - no的HC-Pro顺子；其中30个在感染中功能相容，尽管核苷酸和氨基酸序列分别为55.6%和48.9%，并且（ii）来自SMV-N和ClYVV-No的HC-Pro顺性子。其中30种分别不是栽培大豆或蚕豆宿主特异性的决定因素。
Soybean mosaic virusandClover yellow vein virusare two definite species of the genusPotyviruswithin the familyPotyviridae. Soybean mosaic virus-N (SMV-N) is well adapted to cultivated soybean (Glycine max) genotypes and wild soybean (G. soja), whereas it remains undetectable in inoculated broad bean (Vicia faba). In contrast, clover yellow vein virus No. 30 (ClYVV-No. 30) is capable of systemic infection in broad bean and wild soybean; however, it infects cultivated soybean genotypes only locally. In this study, SMV-N was shown to also infect broad bean locally; hence, broad bean is a host for SMV-N. Based on these observations, it was hypothesized that lack of systemic infection by SMV-N in broad bean and by ClYVV-No. 30 in cultivated soybean is attributable to the incompatibility of multifunctional helper-component proteinase (HC-Pro) in these hosts. The logic of selecting the HC-Pro cistron as a target is based on its established function in systemic movement and being a relevant factor in host range specificity of potyviruses. To test this hypothesis, chimeras were constructed with precise exchanges of HC-Pro cistrons between SMV-N and ClYVV-No. 30. Upon inoculation, both chimeras were viable in infection, but host range specificity of the recombinant viruses did not differ from those of the parental viruses. These observations suggest that (i) HC-Pro cistrons from SMV-N and ClYVV-No. 30 are functionally compatible in infection despite 55.6 and 48.9% nucleotide and amino acid sequence identity, respectively, and (ii) HC-Pro cistrons from SMV-N and ClYVV-No. 30 are not the determinants of host specificity on cultivated soybean or broad beans, respectively.