Mitotically heritable, RNA polymerase II-independent H3K4 dimethylation stimulates INO1 transcriptional memory.
Mitotically heritable, RNA polymerase II-independent H3K4 dimethylation stimulates INO1 transcriptional memory.
复制标题
有丝分裂性的RNA聚合酶II独立的H3K4二甲基化刺激INO1转录记忆。
DOI：
10.7554/elife.77646
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发表时间：
2022-05-17
期刊：
影响因子：
7.7
通讯作者：
Brickner, Jason H.
中科院分区：
文献类型：
--
作者：
Sump, Bethany;Brickner, Donna G.;D'Urso, Agustina;Kim, Seo Hyun;Brickner, Jason H.
对于一些可诱导基因，转录激活的速度和分子机制取决于细胞的先验经验。这种现象被称为表观遗传转录记忆，它加速了再激活，需要染色质结构的改变和稳定的RNA聚合酶II(RNAPII)对启动子的募集。芽期酵母中肌醇饥饿的记忆涉及转录因子依赖的与核孔复合体的相互作用和组蛋白H3赖氨酸4二甲基化(H3K4me2)之间的正反馈循环。虽然H3K4me2对于RNAPII的招募和更快的重新激活是必不可少的，但H3K4me2不需要RNAPII。与依赖RNAPII的H3K4me2与转录相关不同，RNAPII依赖的H3K4me2需要Paf1复合体的Leo1亚单位Nup100，SET3C，并且在必要的转录因子降解时，通过多个细胞周期遗传。该标记的写入者(COMPASS)与潜在的读取器(SET3C)物理上相互作用，提示了H3K4me2在DNA复制后传播和重新结合的分子机制。
For some inducible genes, the rate and molecular mechanism of transcriptional activation depend on the prior experiences of the cell. This phenomenon, called epigenetic transcriptional memory, accelerates reactivation, and requires both changes in chromatin structure and recruitment of poised RNA polymerase II (RNAPII) to the promoter. Memory of inositol starvation in budding yeast involves a positive feedback loop between transcription factor-dependent interaction with the nuclear pore complex and histone H3 lysine 4 dimethylation (H3K4me2). While H3K4me2 is essential for recruitment of RNAPII and faster reactivation, RNAPII is not required for H3K4me2. Unlike RNAPII-dependent H3K4me2 associated with transcription, RNAPII-independent H3K4me2 requires Nup100, SET3C, the Leo1 subunit of the Paf1 complex and, upon degradation of an essential transcription factor, is inherited through multiple cell cycles. The writer of this mark (COMPASS) physically interacts with the potential reader (SET3C), suggesting a molecular mechanism for the spreading and re-incorporation of H3K4me2 following DNA replication.