Palatal mucoperiosteal wound healing in the rat
Palatal mucoperiosteal wound healing in the rat
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DOI：
10.1046/j.0909-8836.1999.eos107505.x
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发表时间：
1999-10-01
影响因子：
1.9
通讯作者：
Kuijpers-Jagtman, AM
中科院分区：
文献类型：
--
作者：
Cornelissen, AMH;Maltha, JC;Kuijpers-Jagtman, AM
本研究的目的是分析未成熟大鼠腭部创伤愈合过程中组织结构和基质成分的变化，并与未接受粘骨膜创伤的同龄大鼠进行比较。在35d龄大鼠上颌骨粘骨膜处制作创面。在损伤后的多个时间点对标本进行组织学评估。分别于伤后8、15、30、60d测定DNA、羟脯氨酸和硫酸氨基糖含量。8d龄大鼠的肉芽组织羟脯氨酸含量比43日龄大鼠减少43%，而糖胺多糖和细胞的含量比43日龄大鼠未损伤的腭部组织多100%。与95天龄大鼠的未损伤组织相比，60天龄大鼠伤口组织的DNA含量增加了100%，羟脯氨酸含量增加了39%。同时，胶原纤维密集排列，横向排列。结论：35d龄大鼠腭部粘骨膜创面愈合时有明显的瘢痕组织形成。与同龄大鼠未损伤的腭部组织相比，瘢痕中含有更多的胶原蛋白。因此，该模型可用于开发旨在减少腭部瘢痕形成的治疗方法。
The objective of this study was to analyze the changes in tissue architecture and matrix composition during healing of palatal wounds of immature rats, and to compare this with rats of the same age that did not receive mucoperiosteal wounds. Wounds were made in the mucoperiosteum of the palate of 35-d-old rats. Samples were evaluated histologically at numerous points in time after wounding. The DNA, hydroxyproline and sulphated glycosaminoglycan contents were determined at 8, 15, 30, and 60 d post-wounding. Eight-d-old granulation tissue contained 43% less hydroxyproline, and 100% more glycosaminoglycans and cells than unwounded palatal tissue of 43-d-old rats. Sixty-d-old wounds contained 100% more DNA and 39% more hydroxyproline than unwounded tissue of 95-d-old rats. At the same time, densely packed and transversely aligned collagen fibres were present. It is concluded that palatal mucoperiosteal wounds made in 35-d-old rats heal with distinct scar tissue formation. The scar contains more collagen than nonwounded palatal tissue of rats of the same age. Therefore, this model may be of use for the development of therapies aiming to reduce palatal scarring.