Suppression of lipopolysaccharide-induced activation of RAW 264.7 macrophages by Se-methylseleno-l-cysteine
Suppression of lipopolysaccharide-induced activation of RAW 264.7 macrophages by Se-methylseleno-l-cysteine
复制标题
Se-甲基硒代-L-半胱氨酸抑制脂多糖诱导的 RAW 264.7 巨噬细胞活化
DOI：
10.1016/j.intimp.2020.107040
复制
发表时间：
2020
影响因子：
5.6
通讯作者：
Hui Wu
中科院分区：
文献类型：
--
作者：
Guang Wang;Qiping Zhan;Hui Wu
Se-methylseleno-l-cysteine (l-SeMC) 是人类有机硒的天然来源。尽管它的结构与l-半胱氨酸（l-Cys）相似，但其抗炎特性和可能的潜在机制尚未被探索。在这里，我们比较了无机硒（亚硒酸盐）、l-Cys 和 l-SeMC 在脂多糖（LPS）激活的 RAW 264.7 小鼠巨噬细胞中的抗炎活性，并重点关注相关的分子和生化事件。结果表明，与单独的l-Cys治疗和l-Cys/亚硒酸盐组合相比，l-SeMC的抗炎活性要强得多。硒的有机形式可能在l-SeMC 的作用中发挥至关重要的作用。进一步研究证实l-SeMC以浓度依赖性方式抑制iNOS、TNF-α、IL-1β、IL-6、COX-2和MMP-9的RNA表达，以及LPS激活的RAW264.7巨噬细胞中NO、TNF-α、IL-6、IL-12p70、COX-2和PGE2的释放。此外，l-SeMC 可以防止 LPS 诱导的细胞形态变化。l-SeMC 浓度在 50 至 200 μM 之间时表现出的抗炎作用接近于 20 μM 地塞米松的抗炎作用。我们的结果表明，l-SeMC 有效抑制 LPS 诱导的 RAW 264.7 巨噬细胞的活化，表明 l-SeMC 可能成为预防或治疗炎症性疾病的潜在功能性食品成分。
Se-methylseleno-l-cysteine (l-SeMC) is a natural source of organic selenium for humans. Although it has a structure similar to that ofl-Cysteine (l-Cys), its anti-inflammatory properties and possible underlying mechanisms have not been explored. Here, we compared the anti-inflammatory activities of inorganic selenium (selenite),l-Cys, andl-SeMC in lipopolysaccharide (LPS)-activated RAW 264.7 murine macrophages and focused on the related molecular and biochemical events. The results showed that, anti-inflammatory activity ofl-SeMC was much stronger compared to both individuall-Cys treatment andl-Cys/selenite combinations. The organic form of selenium may play a crucial role in the effects ofl-SeMC. Further study confirmed thatl-SeMC suppressed the RNA expression ofiNOS,TNF-α,IL-1β,IL-6,COX-2, andMMP-9, as well as the release of NO, TNF-α, IL-6, IL-12p70, COX-2, and PGE2 from LPS-activated RAW264.7 macrophages in a concentration-dependent manner. Moreover,l-SeMC prevented LPS-induced changes in cell morphology.l-SeMC concentrations between 50 and 200 μM exhibited an anti-inflammatory effect closed to that exhibited by 20 μM dexamethasone. Our results demonstrated thatl-SeMC effectively inhibited the activation of RAW 264.7 macrophages induced by LPS, and suggested thatl-SeMC could be a potential functional food component for the prevention or treatment of inflammatory diseases.