Mechanical properties, drug eluting characteristics and in vivo performance of a genipin-crosslinked chitosan polymeric stent
Mechanical properties, drug eluting characteristics and in vivo performance of a genipin-crosslinked chitosan polymeric stent
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DOI：
10.1016/j.biomaterials.2009.06.039
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发表时间：
2009-10-01
期刊：
影响因子：
14
通讯作者：
Sung, Hsing-Wen
中科院分区：
文献类型：
--
作者：
Chen, Mei-Chin;Liu, Chin-Tang;Sung, Hsing-Wen
使用聚合物作为支架基质的限制是其固有的机械弱点。在这项研究中，开发了一种聚合物支架，由具有环状分子结构的京尼平固定的壳聚糖基膜制成（京尼平支架）。京尼平支架的机械性能进行了研究，其对应物固定的线性环氧化合物（环氧树脂支架）和市售的金属支架作为对照。结果表明，京尼平支架基质中形成的环状交联结构有利于其力学性能的提高。此外，京尼平支架的可耐受压缩载荷上级对照金属支架。京尼平支架的细胞毒性显著低于环氧树脂支架。使用French鞘管在家兔肾下腹主动脉中展开京尼平支架。术后3个月，取出的动脉保持通畅;未观察到血栓形成。在植入支架的血管壁上观察到几乎完整的内皮细胞层。为了评估其作为药物递送载体的可能性，将西罗莫司（一种抗增殖药物）装载在京尼平支架中。结果表明，京尼平肝素涂层支架具有线性缓释特性，释放的西罗莫司仍具有抑制平滑肌细胞增殖的活性。这些发现表明，京尼平支架具有增强的机械强度，可用作有吸引力的支架平台，用于局部药物递送。(C)2009爱思唯尔有限公司保留所有权利。
A limitation with the use of polymers as stent matrices is their inherent mechanical weakness. In this study, a polymeric stent, made from chitosan-based films fixed by genipin which has a cyclic molecular structure, was developed (the genipin stent). The mechanical properties of the genipin stent were investigated; its counterpart fixed by a linear epoxy compound (the epoxy stent) and a commercially available metallic stent were used as controls. The results indicated that the cyclic crosslinking structures formed within the genipin stent matrix were beneficiary to the improvement of its mechanical property. Additionally, the tolerable compression load of the genipin stent was superior to that of the control metallic stent. The cytotoxicity of the genipin stent was significantly lower than the epoxy stent. The deployment of the genipin stent in rabbit infrarenal abdominal aortas was performed using a French sheath. At 3 months postoperatively, the retrieved arteries remained patent; no thrombosis was observed. A nearly intact layer of endothelial cells was seen on the stent-implanted vessel wall. To evaluate its possibility as a drug delivery vehicle, sirolimus (an anti-proliferative drug) was loaded in the genipin stent. It was found that the genipin stent with heparin coating exhibited a linear sustained-release profile and the released sirolimus still possessed its original activity in inhibiting smooth muscle cell proliferation. These findings suggest that the genipin stent with enhanced mechanical strength can be used as an attractive stent platform for local drug delivery. (C) 2009 Elsevier Ltd. All rights reserved.