Visualizing cell extracellular matrix (ECM) deposited by cells cultured on aligned bacteriophage M13 thin films.
Visualizing cell extracellular matrix (ECM) deposited by cells cultured on aligned bacteriophage M13 thin films.
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DOI：
10.1021/la201580v
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发表时间：
2011-07
期刊：
影响因子：
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通讯作者：
Laying Wu;L. Andrew Lee;Z. Niu;S. Ghoshroy;Qian Wang
中科院分区：
文献类型：
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作者：
Laying Wu;L. Andrew Lee;Z. Niu;S. Ghoshroy;Qian Wang
从微米到纳米的形貌特征可以影响细胞方向和迁移途径，这是组织工程和肿瘤迁移的重要因素。在我们之前的研究中，噬菌体 M13 的对流组装产生了薄膜，可用于控制细胞的排列。然而，关于其决定细胞排列的根本原因的几个问题仍未得到解答。在这里，我们进一步研究噬菌体 M13 结晶膜产生的纳米形貌特征，从而导致细胞和细胞外基质 (ECM) 蛋白的排列。使用扫描电子显微镜和免疫荧光显微镜记录了排列的细胞和纤维基质蛋白的微米和纳米级的连续成像分析。结果，我们观察到幼仓鼠肾细胞在有序 M13 底物上的排列程度高于 NIH-3T3 成纤维细胞，这种差异可能归因于细胞产生 ECM 蛋白的内在性质。这项研究的结果为生物薄膜的形貌特征提供了重要的见解，可用于控制细胞和周围 ECM 蛋白的方向。
Topographical features ranging from micro- to nanometers can affect cell orientation and migratory pathways, which are important factors in tissue engineering and tumor migration. In our previous study, a convective assembly of bacteriophage M13 resulted in thin films which could be used to control the alignment of cells. However, several questions regarding its underlying reasons to dictate cell alignment remained unanswered. Here, we further study the nanometer topographical features generated by the bacteriophage M13 crystalline film, which results in the alignment of the cells and extracellular matrix (ECM) proteins. Sequential imaging analyses at micro- and nanoscale levels of aligned cells and fibrillar matrix proteins were documented using scanning electron microscopy and immunofluorescence microscopy. As a result, we observed baby hamster kidney cells with higher degree of alignment on the ordered M13 substrates than NIH-3T3 fibroblasts, a difference which could be attributed to the intrinsic nature of the cells' production of ECM proteins. The results from this study provide a crucial insight into the topographical features of a biological thin film, which can be utilized to control the orientation of cells and surrounding ECM proteins.