Interaction of the chick oviduct progesterone receptor with deoxyribonucleic acid.
Interaction of the chick oviduct progesterone receptor with deoxyribonucleic acid.
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鸡输卵管孕酮受体与脱氧核糖核酸的相互作用。
DOI：
10.1021/bi00512a019
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
O'Malley,BW
中科院分区：
文献类型：
--
作者：
Hughes,MR;Compton,JG;Schrader,WT;O'Malley,BW
Mark R. Hughes、1 John G. Compton、5 William T. Schrader、* 和 Bert W. O’Malley 摘要：鸡输卵管黄体酮受体的纯化 DNA 结合成分（受体 A）与克隆的卵清蛋白基因和各种结构组成的质粒 DNA 结合的能力已被分析。 Riggs 等人的快速平衡过滤吸附测定[Riggs, A. D., Suzuki, H., & Bourgeois, S.(1970) J. Mol.生物。 [48, 67] 已用于证明蛋白质与 DNA 的高亲和力结合（Kdiss= 10~ 10 M at 50 mM KCl, pH 7.2）。关联率的研究与平衡测量一致（ti/2=40-80 分钟）。纯化受体与 DNA 的关联以及相互作用的动力学已通过速度沉降技术独立验证。使用卵清蛋白结构基因（cDNA）进行直接结合测定，整个天然卵清蛋白基因包含七个插入序列，并且
Mark R. Hughes, 1 John G. Compton, 5 William T. Schrader,* and Bert W. O’Malley abstract: The purified DNA binding component (receptor A) of the chick oviduct progesterone receptor has been ana-lyzed for its ability to bind to the cloned ovalbumin gene and to plasmid DNA of various structural compositions. The rapid equilibrium filter adsorption assay of Riggs et al.[Riggs, A. D., Suzuki, H., & Bourgeois, S.(1970) J. Mol. Biol. 48, 67] has been used to demonstrate high affinity binding of the protein to DNA {Kdiss= 10~ 10 M at 50 mM KC1, pH 7.2). Studies of association rates are consistent with equilibrium measurements {ti/2=40-80 min). Association of purified receptor with DNA and the kinetics of the interaction have been verified independently by velocity sedimentation techniques. Direct binding assays were performed with the ovalbumin structural gene (cDNA), the entire natural oval-bumin gene containing seven intervening sequences, and