Fresh, dried or smoked? Repellent properties of volatiles emitted from ethnomedicinal plant leaves against malaria and yellow fever vectors in Ethiopia.
Fresh, dried or smoked? Repellent properties of volatiles emitted from ethnomedicinal plant leaves against malaria and yellow fever vectors in Ethiopia.
复制标题
DOI：
10.1186/1475-2875-10-375
复制
发表时间：
2011-12-19
期刊：
影响因子：
3
通讯作者：
Hill SR
中科院分区：
文献类型：
--
作者：
Dube FF;Tadesse K;Birgersson G;Seyoum E;Tekie H;Ignell R;Hill SR
在寻找植物为基础的驱蚊剂，挥发性散发物进行了调查，从五种植物，Corymbia citriodora，罗勒，罗勒lamiifolium，油橄榄和Ostostegia integrifolia，传统上用于在埃塞俄比亚作为保护蚊子。两种蚊子，疟疾媒介阿拉伯按蚊和虫媒病毒媒介埃及伊蚊的行为进行了评估，对从新鲜和干燥的叶子的顶部空间收集的挥发物，以及在两个选择的着陆生物测定和人类气味的背景下燃烧干燥的叶子的烟雾。从燃烧的干树叶的烟雾中的挥发性提取物被发现是更排斥比那些从新鲜的叶子，这反过来又是更排斥蚊子比干树叶的挥发物。在所有的烟雾和新鲜挥发性提取物中，来自柠檬酸的那些（52-76%）和Oc。其中，sweet（58-68%）的驱避性最强，Ol.欧洲楝（23-40%）和全缘叶木（19-37%）的忌避性最低。一个挥发性存在于每个新鲜叶提取物的Co. citriodora，Oc。sweet和Os. integrifolia为假罗勒烯。罗勒烯的含量反映了这三种鲜叶提取物的驱蚊活性。雌性寄主蚊子对罗勒烯的反应呈剂量依赖性，在生理和行为上，在14%的罗勒烯下具有最大的行为排斥。在我们的6分钟着陆试验中，β-罗勒烯（14%）的驱蚊效果与合成驱蚊剂N，N-二乙基-间甲苯酰胺（10% DEET）相当。挥发物在燃烧的烟雾以及新鲜的树叶。甜高粱对寻找An寄主具有显著的驱避特性。arabiensis和Ae.埃及蚊子。在柠檬树的新鲜叶子顶部空间中存在的反罗勒烯，Oc。在实验室条件下，对挥发性驱蚊剂的驱蚊效果显著。除了其驱蚊特性之外，β-罗勒烯长期以来被批准安全地用于食品和化妆品中，使得这种挥发性化合物成为一种有趣的化合物，可以在实验室和现场进行进一步的测试，以验证其驱蚊活性和用于商业产品的潜力。此外，使用人源化膜的着陆生物测定是一种潜在有用的驱虫剂筛选技术，不需要将人类暴露于载体，但建议与常规技术并行进行进一步测试。
In the search for plant-based mosquito repellents, volatile emanations were investigated from five plant species, Corymbia citriodora, Ocimum suave, Ocimum lamiifolium, Olea europaea and Ostostegia integrifolia, traditionally used in Ethiopia as protection against mosquitoes. The behaviour of two mosquitoes, the malaria vector Anopheles arabiensis and the arbovirus vector Aedes aegypti, was assessed towards volatiles collected from the headspace of fresh and dried leaves, and the smoke from burning the dried leaves in a two-choice landing bioassay and in the background of human odour. Volatile extracts from the smoke of burning dried leaves were found to be more repellent than those from fresh leaves, which in turn were more repellent to mosquitoes than volatiles from dried leaves. Of all smoke and fresh volatile extracts, those from Co. citriodora (52-76%) and Oc. suave (58-68%) were found to be the most repellent, Os. integrifolia (29-56%) to be intermediate while Ol. europaea (23-40%) and Os. integrifolia (19-37%) were the least repellent. One volatile present in each of the fresh leaf extracts of Co. citriodora, Oc. suave and Os. integrifolia was ß-ocimene. The levels of ß-ocimene reflected the mosquito repellent activity of these three fresh leaf extracts. Female host-seeking mosquitoes responded dose-dependently to ß-ocimene, both physiologically and behaviourally, with a maximal behavioural repulsion at 14% ß-ocimene. ß-ocimene (14%) repels mosquitoes in our 6-minute landing assays comparable to the synthetic insect repellent N,N-diethyl-m-toluamide (10% DEET). Volatiles in the smoke of burning as well as fresh leaves of Co. citriodora and Oc. suave have significant repellent properties against host seeking An. arabiensis and Ae. aegypti mosquitoes. ß-ocimene, present in the fresh leaf headspace of Co. citriodora, Oc. suave and Os. integrifolia, is a significantly effective volatile mosquito repellent in the laboratory. In addition to its repellent properties, ß-ocimene has long approved safe for use in food and cosmetics, making this volatile an intriguing compound to pursue in further tests in the laboratory and field to validate its mosquito repellent activity and potential for use in a commercial product. Also, the landing bioassay with humanised membranes is a potentially useful repellent screening technique that does not require the exposure of humans to the vectors, however further tests in parallel with conventional techniques are advised.