Physiological roles of endogenous nitric oxide in lymphatic pump activity of rat mesentery in vivo.
Physiological roles of endogenous nitric oxide in lymphatic pump activity of rat mesentery in vivo.
复制标题
内源性一氧化氮在体内大鼠肠系膜淋巴泵活性中的生理作用。
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
T. Ohhashi
中科院分区：
文献类型：
--
作者：
Y. Shirasawa;F. Ikomi;T. Ohhashi
用活体视频显微镜系统研究了内源性一氧化氮（NO）在大鼠肠系膜淋巴泵活动中的生理作用。用显微镜系统测量肠系膜淋巴微血管泵血频率（F）、舒张末期内径（EDD）和收缩末期内径（ESD）的变化，计算泵血流量指数（PFI）。在肠系膜中灌注30 μ M N（ω）-硝基-L-精氨酸甲酯（L-NAME）15分钟，导致F和PFI显著增加，EDD和ESD显著降低。同时灌注1 mM L-精氨酸和30 μ M L-NAME可显著逆转L-NAME介导的F增加和ESD减少。100 μ M氨基胍灌流15分钟对体内肠系膜淋巴管的F、EDD和ESD无显著影响。这些研究结果表明，内源性NO生理调节淋巴泵在大鼠肠系膜在体内的活动，NO的产生和释放可能是由组成型NO合酶介导的，但不是由诱导型NO合酶。
Physiological roles of endogenous nitric oxide (NO) in the lymphatic pump activity of rat mesenteries in vivo were evaluated using an intravital video microscope system. Changes in the pumping frequency (F), the end diastolic diameter (EDD), and the end systolic diameter (ESD) of the mesenteric lymph microvessels were measured with the microscope system and then the pump flow index (PFI) was calculated. A 15-min superfusion of 30 microM N(omega)-nitro-L-arginine methyl ester (L-NAME) in the mesenteries caused significant increases of F and PFI and a significant decrease of the EDD and ESD. Simultaneous superfusion of 1 mM L-arginine with 30 microM L-NAME produced a significant reversal of the L-NAME-mediated increase of F and decrease of ESD. A 15-min superfusion of 100 microM aminoguanidine caused no significant effects on F, EDD, and ESD of the mesenteric lymph vessels in vivo. These findings suggest that endogenous NO has physiologically modulated the lymphatic pump activity in rat mesentery in vivo and that the production and release of NO may be mediated by constitutive NO synthase but not by inducible NO synthase.
影响因子：
20.1
作者：
KUROSE, I;KUBES, P;GRANGER, DN
通讯作者：
GRANGER, DN
影响因子：
--
作者：
BENOIT, JN;ZAWIEJA, DC;GRANGER, HJ
通讯作者：
GRANGER, HJ
DOI：
10.1152/ajpregu.1998.274.3.r790
发表时间：
1998-03-01
影响因子：
2.8
作者：
Mizuno, R;Koller, A;Kaley, G
通讯作者：
Kaley, G