AN ELECTRON MICROSCOPE STUDY OF TOXOPLASMA
AN ELECTRON MICROSCOPE STUDY OF TOXOPLASMA
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DOI：
10.4269/ajtmh.1954.3.1008
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发表时间：
1954-01-01
影响因子：
3.3
通讯作者：
CRAMER, DI
中科院分区：
文献类型：
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作者：
GUSTAFSON, PV;AGAR, HD;CRAMER, DI
弓形虫薄片的电子显微照片揭示了形态细节，这是传统技术研究迄今未曾怀疑的。感染的小鼠腹腔液用OsO4固定，包埋在甲基丙烯酸酯中，用玻璃刀和改良的Spencer旋转切片机进行切片。展示了与细胞的极性组织有关的元素。锐端显示一个非常明显的细胞器，是一个中空的截形圆锥体0.15-0.25[亩]。直径0.2-0.3亩。长。这种结构被称为“二次曲面”。在圆锥体的一端，沿着细胞的纵向路线，有几个长的、大致圆柱体的致密、均匀的物质。最大直径为0.08-0.12[亩]。当它们接近圆锥体时，它们变得非常细长和弯曲，可以看到它们会聚在一起，进入圆锥体的底部。对于这些结构，建议使用“毒素”一词。还描述了各种其他细胞质内含物。细胞核有一层明确的核膜。
Electron micrographs of thin sections of Toxoplasma reveal morphological details hitherto unsuspected from study by traditional techniques. Infected mouse peritoneal fluid was fixed with OsO4, imbedded in methacrylate, and sectioned using glass knives and a modified Spencer rotary microtome. Elements involved in the polar organization of the cells were demonstrated. The acute end shows a very distinct organelle, which is a hollow, truncate cone 0.15-0.25[mu]. in diameter and 0.2-0.3[mu]. long. This structure is referred to as the "conoid." Associated at one end with the conoid and following a longitudinal course through the cell are several long, roughly cylindrical bodies of a dense, homogeneous material. Maximum diameters are 0.08-0.12[mu]. As they approach the conoid, they become very slender and tortuous, and can be seen to converge, entering into the base of the cone. The term "toxoneme" is suggested for these structures. A variety of other cytoplasmic inclusions are described. The nucleus is shown to have a definite nuclear membrane.