ER stress-mediated cell damage contributes to the release of EDA+ fibronectin from hepatocytes in nonalcoholic fatty liver disease
ER stress-mediated cell damage contributes to the release of EDA+ fibronectin from hepatocytes in nonalcoholic fatty liver disease
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内质网应激介导的细胞损伤有助于非酒精性脂肪肝中肝细胞释放 EDA 纤连蛋白
DOI：
10.1007/s11596-017-1718-8
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发表时间：
2017-04
期刊：
影响因子：
--
通讯作者：
Zhiguo Liu
中科院分区：
文献类型：
--
作者：
Ting Chen;Qiang Huang;Yu Wang;Zhiguo Liu
SummaryFibronectin containing extra domain A (EDA+ FN), a functional glycoprotein participating in several cellular processes, correlates with chronic liver disease. Herein, we aim to investigate the expression and secretion of EDA+ FN from hepatocytes in nonalcoholic fatty liver disease (NAFLD) and the underlying mechanisms. Circulating levels of EDA+ FN were determined by ELISA in clinical samples. Western blotting and flow cytometry were performed on L02 and HepG2 cell lines to analyze whether the levels of EDA+ FN were associated with endoplasmic reticulum (ER) stress-related cell death. Circulating levels of EDA+ FN in NAFLD patients were significantly higher than those in control subjects, and positively related with severity of ultrasonographic steatosis score. In cultured hepatocytes, palmitate up-regulated the expression of EDA+ FN in a dose-dependent manner. Conversely, when the cells were pretreated with 4-phenylbutyrate, a specific inhibitor of ER stress, up-regulation of EDA+ FN could be abrogated. Moreover, silencing CHOP by shRNA enhanced the release of EDA+ FN from hepatocytes following palmitate treatment, which was involved in ER stress-related cell damage. These findings suggest that the up-regulated level of EDA+ FN is associated with liver damage in NAFLD, and ER stress-mediated cell damage contributes to the release of EDA+ FN from hepatocytes.
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DOI：
10.1097/mpg.0b013e31821b4b61
发表时间：
2011-08
影响因子：
2.9
作者：
Shannon A;Alkhouri N;Carter-Kent C;Monti L;Devito R;Lopez R;Feldstein AE;Nobili V
通讯作者：
Nobili V
影响因子：
7.3
作者：
Thwaites R;Chamberlain G;Sacre S
通讯作者：
Sacre S
影响因子：
5.4
作者：
Li, Qi;Liu, Zhiguo;Qu, Shen
通讯作者：
Qu, Shen
影响因子：
5
作者：
De-cui Shao;Jun Ni;Yang Shen;Jia Liu;Li Zhou;H. Xue;Yu Huang;Wei Zhang-;Li-min Lu
通讯作者：
De-cui Shao;Jun Ni;Yang Shen;Jia Liu;Li Zhou;H. Xue;Yu Huang;Wei Zhang-;Li-min Lu
影响因子：
3.8
作者：
Attallah, Abdelfattah M.;Abdallah, Sanaa O.;Shaker, Yehia M.
通讯作者：
Shaker, Yehia M.