Differential Inducibility of Human and Porcine Dental Pulp-Derived Cells into Odontoblasts
Differential Inducibility of Human and Porcine Dental Pulp-Derived Cells into Odontoblasts
复制标题
DOI：
10.1080/03008200701507909
复制
发表时间：
2007-01
影响因子：
2.9
通讯作者：
A. Tonomura;Y. Sumita;Y. Ando;D. Iejima;H. Kagami;M. Honda;M. Ueda
中科院分区：
文献类型：
--
作者：
A. Tonomura;Y. Sumita;Y. Ando;D. Iejima;H. Kagami;M. Honda;M. Ueda
从培养的牙髓细胞中产生成牙本质细胞的可靠方法尚未建立。在这项研究中，研究了从培养的人和猪的牙髓来源的细胞中分离成牙本质细胞的有效方法，并特别注意了物种差异。培养的人细胞在地塞米松(Dex)和β-甘油磷酸(β-Gly)存在下表现出较低的碱性磷酸酶(ALP)活性。相反，添加1，25-二羟基维生素D3(VitD3)可显著提高ALP活性。在猪细胞中，β-Gly单独或地塞米松和β-Gly联合使用可显著提高碱性磷酸酶的活性，但添加VitD3会降低这一活性。RT-PCR和Western blotting分析表明，三种诱导剂联合作用于人细胞后，骨钙素和牙本质唾液蛋白的表达显著上调。我们认为地塞米松、β-Gly和VitD3的结合对于人牙髓来源的细胞分化为成牙本质细胞是至关重要的。此外，牙髓来源细胞的诱导性存在显著的物种差异。
A robust method for generating odontoblasts from cultured dental pulp cells has not been established. In this study, efficient methods for deriving odontoblasts from cultured human and porcine dental pulp-derived cells were investigated with special attention to species differences. Cultured human cells showed relatively low alkaline phosphatase (ALP) activity in the presence of dexamethasone (Dex) and β-glycerophosphate (β-Gly). In contrast, the addition of 1,25-dihydroxyvitaminD3 (VitD3) significantly increased the ALP activity. In porcine cells, β-Gly alone or a combination of Dex and β-Gly significantly increased ALP activity; however, addition of VitD3 reduced this activity. RT-PCR and Western blotting analysis revealed that the combination of three induction reagents on human cells significantly upregulates the expression of osteocalcin mRNA, and dentin sialoprotein. We propose that the combination of Dex, β-Gly, and VitD3 is critical for differentiation of human dental pulp-derived cells into odontoblasts. In addition, the inducibility of dental pulp-derived cells presented remarkable species differences.