LABELING OF DYING NEURONS BY PEROXIDASE INJECTED INTRA-VASCULARLY IN CHICK-EMBRYOS
LABELING OF DYING NEURONS BY PEROXIDASE INJECTED INTRA-VASCULARLY IN CHICK-EMBRYOS
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DOI：
10.1016/0304-3940(82)90403-7
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发表时间：
1982-01-01
影响因子：
2.5
通讯作者：
CLARKE, PGH
中科院分区：
文献类型：
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作者：
CLARKE, PGH
有三种证据表明，静脉注射辣根过氧化物酶的标记选择性地在发育中的峡部视神经核中死亡的神经元：在未手术的胚胎中，标记的神经元仅在正常神经元死亡期间被发现;一些标记的神经元明显坏死;眼内注射秋水仙碱导致基本上所有的峡部视神经元退化，然后它们几乎都摄取过氧化物酶。通过顶盖损伤使发育中的峡-视核传入神经阻滞增加了神经元的损失，而几乎不影响标记的数量;这意味着由于传入神经阻滞而死亡的神经元要么不摄取过氧化物酶，要么被异常迅速地吞噬。
Three lines of evidence indicate that i.v. injected horseradish peroxidase labels dying neurons selectively in the developing isthmo-optic nucleus: in unoperated embryos, labeled neurons are found only during the period of normal neuronal death; some of the labeled neurons are manifestly necrotic; an intraocular injection of colchicine causes essentially all the isthmo-optic neurons to degenerate, and then they almost all take up peroxidase. Deafferenting the developing isthmo-optic nucleus by a tectal lesion increases the loss of neurons while scarcely affecting the number labeled; this implies that the neurons which die because of the deafferentation either do not take up the peroxidase or are phagocytozed exceptionally rapidly.