Safening activity and metabolism of the safener cyprosulfamide in maize and wheat
Safening activity and metabolism of the safener cyprosulfamide in maize and wheat
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DOI：
10.1002/ps.5801
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发表时间：
2020-03-10
影响因子：
4.1
通讯作者：
Edwards, Robert
中科院分区：
文献类型：
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作者：
Giannakopoulos, George;Dittgen, Jan;Edwards, Robert
背景安全剂通过选择性增强大粒谷类作物的耐受性来扩大现有除草剂的应用范围。虽然它们的活性与增强除草剂代谢有关，但它们的确切作用方式及其作物特异性的原因尚未确定。在这项研究中，我们研究了最近开发的磺酰胺安全剂环丙磺酰胺（CSA）在玉米（玉米）中的选择性。和小麦（Triticum aestivum）的研究，重点探讨了其在两个物种中的吸收、分布和代谢。结果CSA对噻酮卡巴松（TCM）对玉米的伤害有保护作用，但对小麦无保护作用。这与除草剂在玉米中的选择性增强解毒有关。CSA在玉米中的代谢比在小麦中更快，形成了与安全化相关的特定羟基化代谢物。对thensf1突变体甜玉米的研究表明，CSA的羟基化部分由细胞色素P450 CYP81A9介导。然而，化学合成CSA的初级代谢产物，并测试其在玉米中使TCM安全的能力，但当测试时，作为安全剂是无活性的。结论CSA对中药药害的保护作用与其促进除草剂代谢有关。这种选择活性是由于亲本CSA在玉米中而不是在小麦中的特异性识别。随后CSA的快速氧化代谢导致其失活，表明细胞色素P450调节安全剂和除草剂的活性。(c)2020化学工业协会
BACKGROUND Safeners extend the application of existing herbicides by selectively enhancing tolerance in large-grained cereal crops. While their activity is linked to enhanced herbicide metabolism, their exact mode of action and reasons for their crop specificity have yet to be determined. In this study, we investigated the selectivity of the recently developed sulfonamide safener cyprosulfamide (CSA) in maize (Zea maysL.) and wheat (Triticum aestivum), focusing on its uptake, distribution and metabolism in the two species. RESULTS CSA protected maize, but not wheat, from injury by thiencarbazone-methyl (TCM). This correlated with the selective enhanced detoxification of the herbicide in maize. CSA underwent more rapid metabolism in maize than in wheat, with the formation of a specific hydroxylated metabolite correlating with safening. Studies with thensf1mutant sweetcorn line showed that the hydroxylation of CSA was partly mediated by the cytochrome P450 CYP81A9. However, primary metabolites of CSA were chemically synthesised and tested for their ability to safen TCM in maize but when tested were inactive as safeners. CONCLUSION The results of this study suggest that the protection against TCM injury by CSA is linked to enhanced herbicide metabolism. This selective activity is due to the specific recognition of parent CSA in maize but not in wheat. Subsequent rapid oxidative metabolism of CSA led to its inactivation, demonstrating that cytochrome P450s regulate the activity of safeners as well as herbicides. (c) 2020 Society of Chemical Industry