Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles.
Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles.
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分析果蝇飞行肌肉中氧化磷酸化复合物的完整性。
DOI：
10.1016/j.xpro.2021.101021
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发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Owusu-Ansah E
中科院分区：
文献类型：
--
作者：
Murari A;Owusu-Ansah E
果蝇的飞行肌富含线粒体，并已成为研究氧化磷酸化（OXPHOS）复合物如何组装的强大遗传系统。在这里，我们描述了一系列的协议，通过蓝色的天然聚丙烯酰胺凝胶电泳（BN PAGE）分析OXPHOS复合物在果蝇的完整性。我们还包括了在通过BN PAGE分离OXPHOS复合物后通常进行的额外步骤的方案，例如考马斯染色、银染色和凝胶内OXPHOS活性。有关本方案使用和执行的完整详细信息，请参阅。蓝色非变性聚丙烯酰胺凝胶电泳（BN PAGE）分离OXPHOS复合物可以用BN PAGE评估果蝇飞行肌肉中的OXPHOS组装OXPHOS活性可以通过凝胶内活性测定评估果蝇飞行肌肉高度富集线粒体，并且已经成为研究氧化磷酸化（OXPHOS）复合物如何组装的强大遗传系统。在这里，我们描述了一系列的协议，通过蓝色的天然聚丙烯酰胺凝胶电泳（BN PAGE）分析OXPHOS复合物在果蝇的完整性。我们还包括了在通过BN PAGE分离OXPHOS复合物后通常进行的额外步骤的方案，例如考马斯染色、银染色和凝胶内OXPHOS活性。
Drosophila flight muscles are highly enriched with mitochondria and have emerged as a powerful genetic system for studying how oxidative phosphorylation (OXPHOS) complexes are assembled. Here, we describe a series of protocols for analyzing the integrity of OXPHOS complexes in Drosophila via blue native polyacrylamide gel electrophoresis (BN PAGE). We have also included protocols for the additional steps that are typically performed after OXPHOS complexes are separated by BN PAGE, such as Coomassie staining, silver staining, and in-gel OXPHOS activities. For complete details on the use and execution of this protocol, please refer to. Blue native polyacrylamide gel electrophoresis (BN PAGE) separates OXPHOS complexes OXPHOS assembly in Drosophila flight muscles can be assessed with BN PAGE OXPHOS activities can be assessed by in-gel activity assays Drosophila flight muscles are highly enriched with mitochondria and have emerged as a powerful genetic system for studying how oxidative phosphorylation (OXPHOS) complexes are assembled. Here, we describe a series of protocols for analyzing the integrity of OXPHOS complexes in Drosophila via blue native polyacrylamide gel electrophoresis (BN PAGE). We have also included protocols for the additional steps that are typically performed after OXPHOS complexes are separated by BN PAGE, such as Coomassie staining, silver staining, and in-gel OXPHOS activities.
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影响因子：
8.8
作者：
Ulgherait M;Rana A;Rera M;Graniel J;Walker DW
通讯作者：
Walker DW
影响因子：
8.8
作者：
Garcia CJ;Khajeh J;Coulanges E;Chen EI;Owusu-Ansah E
通讯作者：
Owusu-Ansah E
DOI：
10.1083/jcb.202001071
发表时间：
2020-10-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Murari A;Rhooms SK;Goparaju NS;Villanueva M;Owusu-Ansah E
通讯作者：
Owusu-Ansah E
影响因子：
64.5
作者：
Owusu-Ansah E;Song W;Perrimon N
通讯作者：
Perrimon N
DOI：
10.1073/pnas.0803998105
发表时间：
2008-09-23
影响因子：
11.1
作者：
Deng, Hansong;Dodson, Mark W.;Guo, Ming
通讯作者：
Guo, Ming