Phenotypic and molecular analysis of oleate content in the mutant soybean line M23
Phenotypic and molecular analysis of oleate content in the mutant soybean line M23
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DOI：
10.2135/cropsci2004.0664
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发表时间：
2005-09-01
期刊：
影响因子：
2.3
通讯作者：
Sandhu, D
中科院分区：
文献类型：
--
作者：
Alt, JL;Fehr, WR;Sandhu, D
大豆[Glycine max（L.）梅尔。]具有高油酸酯含量的油可用于需要提高氧化稳定性的食品和工业应用。本研究的第一个目的是确定是否可以使用分子选择的Fad 2 -1缺失与中油酸突变株系M23中的ol等位基因，以确定在育种计划中的中油酸个人。第二个目的是确定是否修饰基因影响油酸含量的表型表达的个人纯合子的删除M23和弓箭手，一个品种之间的杂交与正常的油酸含量。根据Southern分析，来自该杂交的88个F-2植株之间的分离令人满意地符合Fad 2 -1缺失的纯合正常（0101）/杂合（0101）/纯合（0101）的比率。基于PCR的标记Fad 2 -1-ol识别出与Southern分析相同的洛尔个体。基于PCR的标记将是一个更快速和劳动强度更低的方法进行分子选择olol个人比Southern分析。洛尔个体的平均油酸含量最高，洛尔个体居中，洛尔个体的平均油酸含量最低。Olol个体间的变异显著，且其分布与Olol和Olol个体间的分布重叠，表明修饰基因对该性状有重要影响。有必要测试洛尔个体的脂肪酸谱，以选择油酸含量最高的个体。
Soybean [Glycine max (L.) Merr.] oil with elevated oleate content would be useful for food and industrial applications that require increased oxidative stability. The first objective of this study was to determine if molecular selection for the Fad2-1 deletion associated with the ol allele in the mid-oleate mutant line M23 could be used to identify mid-oleate individuals in a breeding program. The second objective was to determine if modifying genes affect phenotypic expression of oleate content in individuals homozygous for the deletion from a cross between M23 and Archer, a cultivar with normal oleate content. The segregation among 88 F-2 plants from the cross satisfactorily fit a ratio of 1:24 homozygous normal (0101)/heterozygous (Olol)/homozygous (olol) for the Fad2-1 deletion on the basis of Southern analysis. A PCR-based marker Fad2-1-ol identified the same olol individuals as the Southern analysis. The PCR-based marker would be a more rapid and less labor intensive method for molecular selection of olol individuals than Southern analysis. The olol individuals had the highest mean oleate content, the Olol individuals were intermediate, and the olol individuals had the lowest mean oleate content. There was significant variation among the Olol individuals and their distribution overlapped that of the Olol and Olol individuals, which indicated that modifying genes had an important influence on the trait. It would be necessary to test the fatty acid profile of olol individuals to select those with the highest oleate content.