PULSED NUCLEAR MAGNETIC-RESONANCE STUDIES ON NA-23, LI-7 AND CL-35 BINDING TO HUMAN OXYHEMOGLOBIN AND CARBON MONOXYHEMOGLOBIN
PULSED NUCLEAR MAGNETIC-RESONANCE STUDIES ON NA-23, LI-7 AND CL-35 BINDING TO HUMAN OXYHEMOGLOBIN AND CARBON MONOXYHEMOGLOBIN
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DOI：
10.1016/0022-2836(73)90327-6
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发表时间：
1973-01-01
影响因子：
5.6
通讯作者：
FORSEN, S
中科院分区：
文献类型：
--
作者：
BULL, TE;ANDRASKO, J;FORSEN, S
用脉冲核磁共振研究了7Li，23Na，35Cl离子在不存在和存在人类氧合血红蛋白和碳一氧合血红蛋白的情况下被用来研究离子和大分子的相互作用。弛豫数据显示Cl−与血红蛋白强烈相互作用，但没有提供Na+结合的证据，直到浓度达到0.5m。在Li+浓度为0.1m左右时，得到了相互作用的证据，并在有限的频率范围内跟踪了弛豫速率对频率的依赖关系，试图分离关联时间和键合离子交换速率对弛豫的影响.在Cl−的情况下，平均寿命除以位点数的上限可以设定为约1 × 10− 6秒，下限可以设定为约1 × 10− 8秒。
Pulsed nuclear magnetic resonance studies of the longitudinal (T1) and transverse (T2) quadrupolar relaxation times of7Li,23Na,35Cl ions in the absence and presence of human oxy- and carbon monoxyhaemoglobin have been used to investigate the interaction of the ions and the macromolecule.The relaxation data show that Cl−interacts strongly with the haemoglobin but provide no evidence for binding of Na+up to concentrations of 0.5m. In the case of Li+, evidence for interaction is obtained at concentrations of about 0.1m.The dependence of relaxation rate on frequency was followed over a limited frequency range in an attempt to separate the effects of correlation times and exchange rates of the bonded ions on the relaxation. In the case of Cl−, an upper limit for the mean lifetime divided by the number of sites can be set at about 1 × 10−6second, and a lower limit at about 1 × 10−8second.