Liquiritigenin, an aglycone of liquiritin in Glycyrrhizae radix, prevents acute liver injuries in rats induced by acetaminophen with or without buthionine sulfoximine
Liquiritigenin, an aglycone of liquiritin in Glycyrrhizae radix, prevents acute liver injuries in rats induced by acetaminophen with or without buthionine sulfoximine
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DOI：
10.1016/j.cbi.2006.03.008
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发表时间：
2006-06-10
影响因子：
5.1
通讯作者：
Kim, Sang Geon
中科院分区：
文献类型：
--
作者：
Kim, Young Woo;Ki, Sung Hwan;Kim, Sang Geon
甘草是西方和东方国家最古老、最常用的植物药之一。以前，我们发现甘草苷元（LQ），甘草苷在G。在体外对重金属诱导的细胞毒性具有保护作用。本研究探讨了LQ对乙酰氨基酚（APAP）或APAP+丁噻酚亚砜亚胺（BSO）诱导的急性肝损伤的保护作用。通过血液生物化学和组织病理学评估单独（p.o.）给予从甘草苷的酸水解物纯化的LQ的大鼠的肝损伤。或静脉注射，2-4天）或与五味子中五味子乙素C的合成衍生物4，4 '-二甲氧基-5，6，5'，6-二亚甲二氧基联苯-2，2 '-二羧酸二甲酯（DDB）组合，并暴露于APAP或APAP + BSO。LQ治疗（口服）有效地减少了由单剂量的APAP诱导的肝损伤，如肝坏死和炎症以及血浆丙氨酸转氨酶和乳酸脱氢酶活性的降低所证明的。LQ，当静脉应用时，以更大的效力增强保肝作用。APAP + BSO导致严重的肝损伤，导致死亡。LQ预处理显著减少了增强的肝坏死，降低了死亡率。DDB对APAP或APAP + BSO引起的肝损伤无明显保护作用，但能改善血液生化指标。LQ和DDB联合应用对大鼠有一定的相加保护作用。本研究表明，LQ有效地保护肝脏免受APAP诱导的急性损伤或APAP诱导的GSH缺乏时的严重损伤，表明LQ是G.基数(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Glycyrrhizae radix has been used as one of the oldest and most frequently employed botanicals in both western and oriental countries. Previously, we showed that liquiritigenin (LQ), an aglycone of liquiritin in G. radix, exerts cytoprotective effects against heavy metal-induced toxicity in vitro. This study investigated in vivo protective effects of LQ against acute liver injuries induced by acetaminophen (APAP) or APAP plus buthionine sulfoximine (BSO). Liver injuries were assessed by blood biochemistry and histopathology in rats administered with LQ purified from the acid hydrolyates of liquiritin singly (p.o. or i.v., 2-4 days) or in combination with dimethyl-4,4'-dimethoxy-5,6,5',6-dimethylenedioxybiphenyl-2,2'-dicarboxylate (DDB), a synthetic derivative of Schisandrin C in Fructus shizandrae, and exposed to APAP or APAP + BSO. LQ treatments (oral) effectively decreased liver injuries induced by a single dose of APAP, as evidenced by decreases in hepatic necrosis and inflammation as well as plasma alanine aminotransferase and lactate dehydrogenase activities. LQ, when intravenously applied, enhanced hepatoprotective effect with a greater potency. APAP + BSO led to severe liver injuries, resulting in lethality. LQ pretreatments significantly reduced the potentiated liver necrosis, decreasing mortality. In spite of the improvement in blood biochemistry, DDB failed to protect the liver from injuries induced by APAP or APAP + BSO. Combined treatments of rats with LQ and DDB showed some additive protective effect. The present study demonstrates that LQ efficaciously protects the liver from acute injuries induced by APAP or from APAP-induced severe injuries during GSH deficiency, indicating that LQ is one of the principal cytoprotective components comprised in G. radix. (c) 2006 Elsevier Ireland Ltd. All rights reserved.