Bacteria Associated with Gut Lumen of Camponotus japonicus Mayr
Bacteria Associated with Gut Lumen of Camponotus japonicus Mayr
复制标题
弓背蚁肠腔相关细菌
DOI：
10.1603/en11157
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发表时间：
2011-12-01
影响因子：
1.7
通讯作者：
Wei, Cong
中科院分区：
文献类型：
--
作者：
He, Hong;Chen, Yuanyao;Wei, Cong
Camponotus蚂蚁在其中肠细菌细胞中含有专性细胞内共生体Blochmannia，但对生活在肠腔中的肠道细菌知之甚少。本文报道了应用16 SrRNA聚合酶链反应-限制性片段长度多态性技术对弓背蚁工蚁肠道菌群的研究结果。从107个克隆中，鉴定出11种不同的限制性片段长度多态性谱，序列爆破分析发现这些代表四种类型的细菌。大部分（91.6%）的克隆是Camponotus蚂蚁的专性内共生体“Blochmannia”，6.5%的克隆是蚜虫的次级内共生体“Serratia symbiotica”，其余2%的克隆分别是Frucidococcus fructosus和未培养的Burkholderiales细菌。这些结果表明，C.价格很低。首次从Camponotus蚂蚁中鉴定出“共生沙雷氏菌”，这是一个有趣的结果，因为Blochmannia最接近的细菌亲戚也在沙雷氏菌属中。这一发现支持了蚜虫蜜露或组织的消费提供了一个先进的共生进化的第一步的情况下，并建议Camponotus蚂蚁可以获得其他次生内共生体从半翅目主机通过他们的饮食。此外，还从C.其是否为弓背蚁中的氮循环内共生体有待进一步研究。
Camponotus ants harbor the obligate intracellular endosymbiont Blochmannia in their midgut bacteriocytes, but little is known about intestinal bacteria living in the gut lumen. In this paper we reported the results of a survey of the intestinal microfiora of Camponotus japonicus Mayr based on small-subunit rRNA genes (16S rRNAs) polymerase chain reaction (PCR)-restriction fragment-length polymorphism analysis of worker guts. From 107 clones, 11 different restriction fragment-length polymorphism profiles were identified, and sequences blasting analysis found these represent four types of bacteria. Most (91.6%) of the clones were "Candidatus Blochmannia", the obligate endosymbionts of Camponotus ants, and 6.5% of the clones were "Candidatus Serratia symbiotica", a secondary endosymbiont of aphids; the remaining 2% clones were Fructobacillus fructosus and uncultured Burkholderiales bacterium, respectively. These results show that the diversity of gut bacteria in C. japonicus was low. "Candidatus Serratia symbiotica" was identified from Camponotus ants for the first time, an interesting result because Blochmannia's closest bacterial relative is also in the genus Serratia. This discovery supports the scenario that consumption of aphid honeydew or tissue provides an initial step in the evolution of an advanced symbiosis, and suggests that Camponotus ant could acquire other secondary endosymbionts from Hemiptera host through their diet. In addition, Burkholderiales bacterium also was identified from the gut of C. japonicus for the first time, and whether it is a nitrogen-recycling endosymbiont in Camponotus ants needs to be investigated further.