Functional CD5+ B cells develop predominantly in the spleen of NOD/SCID/γcnull (NOG) mice transplanted either with human umbilical cord blood, bone marrow, or mobilized peripheral blood CD34+ cells
Functional CD5+ B cells develop predominantly in the spleen of NOD/SCID/γcnull (NOG) mice transplanted either with human umbilical cord blood, bone marrow, or mobilized peripheral blood CD34+ cells
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DOI：
10.1016/s0301-472x(03)00193-0
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发表时间：
2003-09-01
影响因子：
2.6
通讯作者：
Habu, S
中科院分区：
文献类型：
--
作者：
Matsumura, B;Kametani, Y;Habu, S
Objective.人CD 5（+）B细胞是胎儿脾脏和脐带血（CB）中主要的B细胞亚群，从婴儿期到儿童期，它们在脾脏和外周血中的数量逐渐减少，而常规B细胞增加。在这项研究中，我们研究了是否CD 5（+）细胞分化成人造血干细胞（HSC）以及在免疫缺陷小鼠胎儿的。在我们的系统中，NOD/SCID/gammac（null）（NOG）小鼠分别移植来自CB（hCB模型）、成人骨髓（hBM模型）和动员外周血（hMPB模型）的CD 34（+）细胞。在这些模型小鼠中，移植后4至8周，无论CD 34（+）细胞来源如何，脾脏中均出现高比例的CD 19（+）IgM（+）CD 5（+）成熟B细胞，而BM中大多数为CD 19（+）IgM（-）CD 5（-）未成熟B细胞。CD 19（+）CD 5（-）BM细胞与NOG脾细胞共培养后，可表达CD 5。DNP-KLH免疫hCB模型小鼠后，血清中抗原特异性IgM增强，而IgG无明显增强。我们的研究结果表明，成人CD 34（+）细胞在NOG脾中发育成功能性CD 5（+）B细胞的数量与胎儿CD 34（+）细胞一样多。(C)2003年国际实验血液学学会。爱思唯尔公司出版
Objective. Human CD5(+) B cells are the major B cell subset in fetal spleen and umbilical cord blood (CB), and their number gradually diminishes in both spleen and peripheral blood from infancy through childhood while conventional B cells increase. In this study, we investigated whether CD5(+) cells differentiate from adult hematopoietic stem cells (HSCs) as well as fetal ones in immunodeficient mice.Methods. In our system, NOD/SCID/gammac(null) (NOG) mice were transplanted with CD34(+) cells from CB (hCB model), adult bone marrow (hBM model), and mobilized peripheral blood (hMPB model).Results. In these model mice, a high proportion of CD19(+)IgM(+)CD5(+) mature B cells appeared in the spleen, regardless of the CD34(+) cell origin, 4 to 8 weeks after transplantation, while the majority were CD19(+)IgM(-)CD5(-) immature B cells in BM. The CD19(+)CD5(-) BM cells showed to express CD5 after the coculture with NOG spleen cells. In the sera of immunized hCB model mice with DNP-KLH, antigen-specific IgM but not IgG was enhanced.Conclusion. Our results show that adult CD34(+) cells develop into functional CD5(+) B cells in NOG spleen as much as fetal CD34(+) cells do. (C) 2003 International Society for Experimental Hematology. Published by Elsevier Inc.