Production of triterpene acids by cell suspension cultures of Olea europaea
Production of triterpene acids by cell suspension cultures of Olea europaea
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DOI：
10.1248/cpb.55.784
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发表时间：
2007-05-01
影响因子：
1.7
通讯作者：
Ebizuka, Yutaka
中科院分区：
文献类型：
--
作者：
Saimaru, Hiroshi;Orihara, Yutaka;Ebizuka, Yutaka
油橄榄（Oleaeuropaea）含有大量的三萜酸，其中齐墩果酸（6）是主要的三萜酸。三萜酸类化合物具有多种生物活性，特别是从药理学的角度，引起了人们的广泛关注。对橄榄植株的细胞培养进行了诱导，并对其三萜成分进行了研究。从细胞悬浮培养物中分离出六种熊烷型三萜酸：熊果酸（9）、波莫酸（10）、罗通酸（11）、苦杏仁酸（12）、2 α-羟基熊果酸（13）和19 α-羟基积雪草酸（14），以及两种齐墩果烷型酸：齐墩果酸和山楂酸（7）。细胞培养产生的三萜酸中，乌索烷型三萜酸的量大于齐墩果烷型三萜酸。此外，一个多功能的氧化角鲨烯环化酶（OSC）命名为OEA从相同的培养细胞的同源性为基础的PCR克隆。OEA的主要产物是α-香树脂醇（熊烷骨架），与培养细胞中较高含量的熊烷型三萜酸表现出良好的一致性，并强烈表明OEA是其生产的主要贡献者OSC。
Olive (Olea europaea) contains large quantity of triterpene acids including oleanollic acid (6) as a major one. Varieties of biological activities exhibited by triterpene acids attracted our attentions, especially from pharmaceutical viewpoints. Cell culture of olive plant was induced and its triterpene constituents were studied. From the cell suspension cultures, six ursane type triterpene acids; ursollic acid (9), pomolic acid (10), rotundic acid (11), tormentic acid (12), 2 alpha-hydroxyursolic acid (13) and 19 alpha-hydroxyasiatic acid (14), and two oleanane type acids; oleanolic acid and maslinic acid (7), have been isolated. Quantity of ursane type triterpene acids produced by cell cultures was larger than that of oleanane type. Further, a multifunctional oxidosqualene cyclase (OSC) named OEA was cloned by homology based PCRs from the same cultured cells. Major product of OEA is alpha-amyrin (ursane skeleton), showing good accordance to higher content of ursane-type triterpene acids in the cultured cells, and strongly suggesting OEA to be a major contributor OSC for their production.