The DUF288 domain containing proteins GhSTLs participate in cotton fiber cellulose synthesis and impact on fiber elongation.
The DUF288 domain containing proteins GhSTLs participate in cotton fiber cellulose synthesis and impact on fiber elongation.
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DOI：
10.1016/j.plantsci.2021.111168
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发表时间：
2021-12
期刊：
影响因子：
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通讯作者：
Yanjun Guo;F. Chen;Jinwen Luo;Mengfei Qiao;W. Zeng;Juan Li;Wenliang Xu
中科院分区：
文献类型：
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作者：
Yanjun Guo;F. Chen;Jinwen Luo;Mengfei Qiao;W. Zeng;Juan Li;Wenliang Xu
棉花是世界上最重要的经济作物之一，成熟纤维中含有90%以上的纤维素。然而，人们对棉纤维中纤维素的合成机制知之甚少。在这里，我们鉴定了四种含有 DUF288 结构域的蛋白质，我们将其命名为 GhSTL1-4。这四个 GhSTL 基因在花后 6 天 (dpa) 和 20 dpa 棉纤维中高度表达。它们定位于高尔基体，可以不同程度地挽救拟南芥stl1stl2double突变体纤维素合成的初生细胞壁（PCW）和次生细胞壁（SCW）生长缺陷。 GhSTL 的沉默导致纤维素含量减少和纤维变短。此外，分裂泛素膜酵母双杂交分析表明，GhSTL1和GhSTL4可以与PCW相关的GhCesA6-1/6-3和SCW相关的GhCesA7-1/7-2相互作用。 GhSTL3 可以与 SCW 相关的 GhCesA4-3 相互作用。这些相互作用通过萤火虫荧光素酶互补成像测定进一步证实。我们共同证明 GhSTL 可以选择性地与 PCW 和 SCW 相关的 GhCesAs 相互作用，并对纤维素合成和纤维发育产生影响。我们的研究结果为棉纤维中纤维素生物合成的机制提供了见解，并提供了协调棉纤维的 PCW 和 SCW 纤维素合成的潜在候选基因，以开发具有增强纤维质量的优良棉花品种。
Cotton is one of the most important economic crops in the world, with over 90 % cellulose in the mature fiber. However, the cellulose synthesis mechanism in cotton fibers is poorly understood. Here, we identified four DUF288 domain containing proteins, which we designated GhSTL1-4. These fourGhSTLgenes are highly expressed in 6 days post anthesis (dpa) and 20 dpa cotton fibers. They are localized to the Golgi apparatus, and can rescue the growth defects in primary cell wall (PCW) and secondary cell wall (SCW) of cellulose synthesis of the Arabidopsisstl1stl2double mutant at varying degrees. Silencing ofGhSTLs resulted in reduced cellulose content and shorter fibers. In addition, split-ubiquitin membrane yeast two-hybrid analysis showed that GhSTL1 and GhSTL4 can interact with PCW-related GhCesA6-1/6-3 and SCW-associated GhCesA7-1/7-2. GhSTL3 can interact with SCW-related GhCesA4-3. These interactions are further confirmed by firefly luciferase complementation imaging assay. Together, we demonstrate that GhSTLs can selectively interact with both the PCW and SCW-associated GhCesAs and impact on cellulose synthesis and fiber development. Our findings provide insights into the mechanism underlying cellulose biosynthesis in cotton fibers, and offer potential candidate genes to coordinate PCW and SCW cellulose synthesis of cotton fibers for developing elite cotton varieties with enhanced fiber quality.