In vitro induction of inflorescence in Dioscorea zingiberensis
In vitro induction of inflorescence in Dioscorea zingiberensis
复制标题
薯蓣花序的体外诱导
DOI：
10.1007/s11240-009-9595-x
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发表时间：
2009-11-01
影响因子：
3
通讯作者：
Yao, Jia-Ling
中科院分区：
文献类型：
--
作者：
Huang, Xiao-Long;Yang, Bo;Yao, Jia-Ling
以盾叶薯蓣为材料，研究了离体培养条件下花序的诱导和再生花的形态建成。H.赖特花序诱导受激素种类和浓度的影响。当花芽外植体在含有2.0毫克/升（-1）6-苄基腺嘌呤和0.5毫克/升（-1）吲哚-3-丁酸组合的Murashige和Skoog培养基上培养时，观察到花序诱导的最高频率。而赤霉酸处理虽能增加花序节长，但诱导效率却降低。个体发生研究表明，花序原基直接起源于7天后的花被和苞片的腋表皮细胞。离体条件下，雄花发育正常，90-100天后开花。此外，还观察到一些两性花。这些结果表明，离体条件下的花芽性别分化与体内条件下的花芽性别分化存在差异。
Inflorescence induction and morphogenesis of regenerated flowers were investigated in vitro in Dioscorea zingiberensis C. H. Wright. Inflorescence induction was influenced by the type and concentration of phytohormones. When floral bud explants were incubated on a Murashige and Skoog medium containing a combination of 2.0 mg l(-1) 6-benzyladenine and 0.5 mg l(-1) indole-3-butyric acid, the highest frequency of inflorescence induction was observed. However, in the presence of gibberellic acid, induction efficiency was reduced although node length of inflorescence was increased. Ontogenetic studies revealed that the inflorescence primordia originated directly from axillary epidermal cells of the perianth and bract of the explants after 7 days. In vitro, male flowers developed normally and blossomed after 90-100 days. In addition, some bisexual flowers were observed. These results demonstrated that there were differences in sexual differentiation of floral buds in vitro compared with that in vivo.