Rapid detection of cytomegalovirus DNA in cerebrospinal fluid of AIDS patients with neurologic disorders.
Rapid detection of cytomegalovirus DNA in cerebrospinal fluid of AIDS patients with neurologic disorders.
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快速检测患有神经系统疾病的艾滋病患者脑脊液中巨细胞病毒 DNA。
DOI：
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发表时间：
1992
影响因子：
6.4
通讯作者：
J. Petit
中科院分区：
文献类型：
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作者：
J. Gozlan;J. Salord;E. Roullet;M. Baudrimont;F. Caburet;O. Picard;M. Meyohas;C. Duvivier;C. Jacomet;J. Petit
应用聚合酶链反应（PCR）技术检测了67例人类免疫缺陷病毒（HIV）感染者的82份脑脊液（CSF）标本中巨细胞病毒（CMV）DNA。14例患者的试验呈阳性，其中8例经脑脊液病毒培养或组织学检查证实为CMV相关神经系统疾病。在PCR阳性的患者中，脑炎是最常见的表现，但在一些周围神经病变或多发性神经根脊髓炎患者中也存在CMV DNA。所有确诊为CMV神经系统疾病的患者PCR均为阳性。相比之下，8名患者中有4名患者的病毒培养呈阴性，仅5名患者的病理学研究可用。由于缺乏参比标准品，无法精确评估基于PCR的检测方法的特异性，但结果与临床病程和其他方法的结果相关性良好。这些结果表明，PCR为基础的方法可能是一个有用的非侵入性工具，快速诊断CMV相关的神经系统疾病。
A polymerase chain reaction (PCR)-based method was used to detect cytomegalovirus (CMV) DNA in 82 cerebrospinal fluid (CSF) samples from 67 patients infected by human immunodeficiency virus (HIV). The test was positive for 14 patients, 8 of whom had CMV-related neurologic disease proven by viral culture of CSF or histologic examination. Encephalitis was the most frequent manifestation in patients with positive PCR results, but CMV DNA was also present in some patients with peripheral neuropathy or polyradiculomyelitis. All patients with proven CMV neurologic disease were positive by PCR. In contrast, viral culture was negative for 4 of the 8 patients and pathologic studies were available only for 5. The specificity of the PCR-based assay could not be assessed precisely because of the lack of a reference standard, but the results correlated well with clinical course and results of the other methods. These findings suggest that the PCR-based method may be a useful noninvasive tool for the rapid diagnosis of CMV-related neurologic disease.