Tebuconazole resistance of Fusarium graminearum field populations from wheat in Henan Province
Tebuconazole resistance of Fusarium graminearum field populations from wheat in Henan Province
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河南省小麦禾谷镰刀菌田间种群戊唑醇抗性
DOI：
10.1111/jph.13021
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发表时间：
2021-06-16
影响因子：
1.5
通讯作者：
Liu, Shengming
中科院分区：
文献类型：
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作者：
Chen, Jinpeng;Wei, Jiangqiao;Liu, Shengming
小麦赤霉病是由禾谷镰刀菌（Fusarium graminearum）引起的一种流行性病害，对作物产量造成严重的经济损失。1，118株F.对2018-2020年从河南省不同地区获得的禾谷镰刀菌菌株，采用5.0 μ g/ml的区分剂量进行测定。共362个F。禾谷镰刀菌对戊唑醇的抗性频率较高，而禾谷镰刀菌对戊唑醇的抗性频率较高。禾谷镰刀菌对戊唑醇的敏感性为30.7%。结果表明，F.根据河南禾谷镰刀菌对戊唑醇的菌丝生长抑制作用计算了禾谷镰刀菌菌株对戊唑醇的敏感性。graminearum种群756株敏感株和362株耐药株的EC_（50）值范围分别为0.01-0.69和0.02-3.31 μ g/ml，敏感株和耐药株的平均± SD EC_（50）值分别为0.13 ± 0.01和0.40 ± 0.13 μ g/ml。756株敏感株和362株耐药株的EC_（90）值范围分别为0.31-4.89和0.65-18.69 μ g/ml，平均值分别为1.13 ± 0.05和2.98 ± 0.09 μ g/ml。用聚合酶链反应（PCR）分别扩增敏感株和耐药株的CYP 51 A和CYP 51 B基因，并进行分析。结果表明，150株耐药菌株中，仅有6株耐药菌株发生氨基酸突变，突变位点为CYP 51 B基因第169位丝氨酸变为酪氨酸（TCA-TAT，Ser-Tyr），其余144株耐药菌株均未发现CYP 51 B基因氨基酸突变。150株耐药菌株的CYP 51 A基因未发现氨基酸突变。本研究结果为进一步监测黄曲霉对戊唑醇的抗药性提供了重要依据。禾谷镰刀菌（graminearum）在河南省小麦田间种群中的分布。
Fusarium head blight (FHB) is caused by Fusarium graminearum, which contributes serious economic losses to crop yields, and is an epidemic disease of wheat. The resistance to tebuconazole of 1,118 F. graminearum strains obtained from different areas of Henan Province in 2018-2020 was determined by the discriminatory doses of 5.0 mu g/ml. A total of 362 F. graminearum strains resistant to tebuconazole were detected, and the frequency of resistance of F. graminearum strains to tebuconazole was 30.7%. The EC50 and EC90 values of F. graminearum strains to tebuconazole were calculated according to the mycelial growth inhibition of the Henan F. graminearum population. The range of EC50 values of 756 sensitive strains and 362 resistant strains were 0.01-0.69 and 0.02-3.31 mu g/ml, and the average +/- SD EC50 of sensitive and resistant strains were 0.13 +/- 0.01 and 0.40 +/- 0.13 mu g/ml. The range of EC90 values of 756 sensitive strains and 362 resistant strains were 0.31-4.89 and 0.65-18.69 mu g/ml, and the average +/- SD EC90 values were 1.13 +/- 0.05 and 2.98 +/- 0.09 mu g/ml. The CYP51A and CYP51B genes of the sensitive and resistant strains were amplified separately by polymerase chain reaction (PCR) and analysed. The results showed that among the 150 resistant strains, only six resistant strains had amino acid mutation, which changed from serine to tyrosine (TCA-TAT, Ser-Tyr) at position 169 of CYP51B gene, and no amino acid mutation was found in CYP51B of the other 144 resistant strains. No amino acid mutation was found in the CYP51A gene of 150 resistant strains. These results provide important information to growers for monitoring tebuconazole resistance in F. graminearum field populations from wheat in Henan Province.