Genomewide association study of Aegilops tauschii traits under seedling-stage cadmium stress
Genomewide association study of Aegilops tauschii traits under seedling-stage cadmium stress
复制标题
苗期镉胁迫下节节山羊草性状的全基因组关联研究
DOI：
10.1016/j.cj.2015.04.005
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发表时间：
2015-10-01
期刊：
影响因子：
6.6
通讯作者：
Liu, Yaxi
中科院分区：
文献类型：
--
作者：
Qin, Peng;Wang, Lang;Liu, Yaxi
节节麦是普通小麦(Triticum Aestivum)的野生近缘种，是抗生物逆境和非生物逆境等优良基因的重要来源。为了提高利用节节麦资源的小麦品种对Cd的耐性，以2 35份节节麦材料为材料，研究了0(对照)和10 0 mU/m ol L-1Cd Cl2(Cd胁迫)处理下节节麦生物量耐性的遗传变异。同时，我们使用包含7185个标记的单核苷酸多态芯片进行了全基因组关联研究。通过一般线性模型和混合线性模型，发现6个标记与Cd耐受性显著相关。根据公共数据库的结果，这些标记与几个与Cd耐性相关的候选/侧翼基因很接近，包括pDIL5-1、ACC-1、DME-5A、TaAP2-D、TaAP2-B、VRN-B1和FtsH样蛋白基因。根据二级指标--综合指数(SI)，将节节麦划分为高度耐镉、中等耐镉和低耐镉三类，其比例分别为9%、57%和34%。从平均SI来看，来自阿富汗、土耳其、阿塞拜疆和伊朗的材料表现出相对较高的Cd耐性。(C)2015年中国作物科学学会和中国科学院作物科学研究所。由爱思唯尔B.V.制作和托管。这是CC By-NC-ND许可证(http://creativecommons.org/licenses/by-nc-nd/4.0/).下的一篇开放获取文章
Aegilops tauschii Ais a wild relative of commonwheat (Triticum aestivum) and acts as an important resource of elite genes including genes for resistance to biotic and abiotic stresses. To improve the cadmium(Cd) tolerance of wheat varieties using A. tauschii resources, we investigated the genetic variation of biomass-based Cd tolerance in 235 A. tauschii accessions treated with 0 (control) and 100 mu mol L-1 CdCl2 (as Cd stress). Simultaneously, we performed a genomewide association study (GWAS) using a single-nucleotide polymorphism chip containing 7185 markers. Six markers were found to be significantly associated with Cd tolerance by a general linear model and a mixed linear model. These markers were close to several candidate/flanking genes associated with Cd tolerance according to results in public databases, including pdil5-1, Acc-1, DME-5A, TaAP2-D, TaAP2-B, Vrn-B1, and FtsH-like protein gene. The A. tauschii accessions were classified as high, moderate, and low Cd-tolerant according to a secondary index, the synthetic index (SI), in proportions of 9%, 57%, and 34%, respectively. By the average SI, accessions from Afghanistan, Turkey, Azerbaijan, and Iran showed relatively high Cd tolerance. (C) 2015 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).