Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species
Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species
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DOI：
10.1038/nprot.2006.227
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发表时间：
2006-01-01
期刊：
影响因子：
14.8
通讯作者：
Lieber, Charles M.
中科院分区：
文献类型：
--
作者：
Patolsky, Fernando;Zheng, Gengfeng;Lieber, Charles M.
生物和化学物种的检测和定量是医疗保健和生命科学的许多领域的核心，从诊断疾病到发现和筛选新药分子。配置为电子器件的半导体纳米线已经成为用于生物和化学物种的超灵敏直接电检测的通用平台。在这里，我们描述了实现纳米线电子传感器的详细协议。首先，生长均匀，单晶硅纳米线，和随后的隔离的纳米线作为稳定的悬浮液概述。其次，描述了可寻址纳米线器件阵列的制造。第三，共价修饰的纳米线器件表面与受体进行了说明。第四，详细描述了来自设备的电响应的示例修改和测量。硅纳米线（SiNW）器件已被证明可用于无标记、超灵敏和高选择性的实时检测各种生物和化学物种，包括蛋白质、核酸、小分子和病毒。
Detection and quantification of biological and chemical species are central to many areas of healthcare and the life sciences, ranging from diagnosing disease to discovery and screening of new drug molecules. Semiconductor nanowires configured as electronic devices have emerged as a general platform for ultra-sensitive direct electrical detection of biological and chemical species. Here we describe a detailed protocol for realizing nanowire electronic sensors. First, the growth of uniform, single crystal silicon nanowires, and subsequent isolation of the nanowires as stable suspensions are outlined. Second, fabrication of addressable nanowire device arrays is described. Third, covalent modification of the nanowire device surfaces with receptors is described. Fourth, an example modification and measurements of the electrical response from devices are detailed. The silicon nanowire (SiNW) devices have demonstrated applications for label-free, ultrasensitive and highly-selective real-time detection of a wide range of biological and chemical species, including proteins, nucleic acids, small molecules and viruses.