Curli expression of enterotoxigenic Escherichia coli.
Curli expression of enterotoxigenic Escherichia coli.
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产肠毒素大肠杆菌的 Curli 表达。
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
T. Pál
中科院分区：
文献类型：
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作者：
E. Szabó;A. Skedsmo;Á. Sonnevend;K. al;L. Emődy;A. Usmani;T. Pál
通过透射电子显微镜和使用卷曲特异性抗体的ELISA检测了104株产肠毒素的广泛地理来源的大肠杆菌菌株的卷曲菌毛表达，并通过PCR检测了卷曲特异性基因序列的存在。所有分离株，无论菌毛的生产，进行特定的结构（csgA）和curli表达的调节基因（crl）之一，分别序列。56株（53.8%）菌株检测到卷曲菌毛。36株菌仅在30 ℃下生长时表达curli，4株菌仅在37 ℃下弱curli，而16株菌在两种温度下均观察到curli。在两种温度下携带curli的分离物上，30 ℃下的菌毛表达显著强于37 ℃。Curli熟练度显著但不完全与刚果红染料的结合相关。curli的表达没有赋予ETEC菌株上皮细胞侵袭性，但是一旦在30摄氏度下表达，它就促进了细菌对塑料表面的粘附。Curli存在于超过一半的ETEC菌株中，并在低温下优先表达，这可能是促进这种食源性和水传播病原体在环境中生存的一个因素。
One hundred and four enterotoxin producing Escherichia coli strains of wide geographical origin were tested for the expression of curli fimbriae by transmission electronmicroscopy and by ELISA using curli-specific antibodies, as well as for the presence of curli-specific gene sequences by PCR. All isolates, irrespective of the production of the fimbriae, carried sequences specific for the structure (csgA) and for one of the regulator genes (crl) of curli expression, respectively. Curli fimbriae were detected in 56 strains (53.8 %). Thirty-six strains expressed curli only when growing at 30 degrees C, 4 isolates were weakly curliated at 37 degrees C only, while on 16 strains curli was observed at both temperatures. On isolates carrying curli at both temperatures the expression of the fimbria was significantly stronger at 30 degrees C than at 37 degrees C. Curli proficiency significantly, but not completely, correlated with the binding of the Congo Red dye. The expression of curli did not confer epithelial cell invasiveness to ETEC strains but, once expressed at 30 degrees C, it facilitated the adherence of the bacteria to plastic surfaces. Curli present in more than half of the ETEC strains and expressed preferentially at low temperatures could be a factor facilitating the environmental survival of this food- and water-borne pathogen.