Expression of antimicrobial peptide genes oscillates along day/night rhythm protecting mice skin from bacteria.
Expression of antimicrobial peptide genes oscillates along day/night rhythm protecting mice skin from bacteria.
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DOI：
10.1111/exd.14229
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发表时间：
2021-10
影响因子：
3.6
通讯作者：
Pyza E
中科院分区：
文献类型：
--
作者：
Bilska B;Zegar A;Slominski AT;Kleszczyński K;Cichy J;Pyza E
抗菌肽是先天免疫系统的重要组成部分，参与皮肤保护和伤口愈合。在此，我们评估了在光/暗循环（LD 12：12）和恒定黑暗（DD）期间小鼠皮肤中chemerin（Rarres 2）、cathelicidin CRAMP（Camp）和三种β-防御素（Defb 1、Defb 3和Defb 14）的基因表达。接下来，我们检查了在一天中的特定时间应用于皮肤上的细菌的存活率。我们发现，Rarres 2，Camp和Defb 1的表达在黑暗开始后4 h最高，在小鼠的高活动期间。然而，这些节奏，并没有保持DD下的皮肤，但存在于肝脏。这表明，在皮肤的情况下，昼夜节律输入被环境中每天的光变化所掩盖。相反，Defb 3和Defb 14在小鼠睡眠时表现出最高的mRNA水平，并且这些有节奏的mRNA振荡在DD下得以维持。这表明皮肤中的Rarres 2，Camp和Defb 1水平与小鼠的高运动活性相关，并且它们受到光照和黑暗的日常变化的控制。另外，Defb 3和Defb 14 mRNA水平的波动似乎可以在睡眠期间保护皮肤和愈合伤口。这些节律在DD下维持，表明它们受到昼夜节律钟的调节。我们的研究表明，每日AMP表达影响皮肤表面细菌的存活，这取决于AMP循环的阶段。
Antimicrobial peptides (AMPs) are important components of the innate immune system and are involved in skin protection against environmental insults and in wound healing. Herein, we assessed the gene expression of chemerin (Rarres2), cathelicidin CRAMP (Camp), and three β-defensins (Defb1, Defb3, and Defb14) in mouse skin during light/dark cycle (LD 12:12) and constant darkness (DD). Next, we examined the survival of bacteria applied on the skin at specific times during the day. We found that the expression of Rarres2, Camp, and Defb1 was the highest at 4 h after the beginning of darkness, during high activity of mice. These rhythms, however, were not maintained under DD in the skin but were present in the liver. This indicated that in the case of skin, a circadian input was masked by daily changes of light in the environment. In contrast, Defb3 and Defb14 showed the highest mRNA levels when the mice slept, and these rhythmic mRNA oscillations were maintained under DD. This shows that Rarres2, Camp, and Defb1 levels in the skin are correlated with high locomotor activity in mice and they are controlled by daily changes of light and dark. Alternatively, oscillations in the mRNA levels of Defb3 and Defb14 seem to protect skin and heal wounds during sleep. These rhythms are maintained under DD, indicating that they are regulated by a circadian clock. Our study suggests that daily AMP expression affects the survival of bacteria on the surface of skin, which depends on the phase of AMP cycling.
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影响因子：
82.9
作者：
通讯作者：
--
影响因子：
3.4
作者：
Jasinska, Malgorzata;Grzegorczyk, Anna;Pyza, Elzbieta
通讯作者：
Pyza, Elzbieta
DOI：
10.1073/pnas.1209592109
发表时间：
2012-07-17
影响因子：
11.1
作者：
Geyfman, Mikhail;Kumar, Vivek;Andersen, Bogi
通讯作者：
Andersen, Bogi
影响因子：
3.7
作者：
Banas M;Zegar A;Kwitniewski M;Zabieglo K;Marczynska J;Kapinska-Mrowiecka M;LaJevic M;Zabel BA;Cichy J
通讯作者：
Cichy J
影响因子：
5.5
作者：
Jann, Naja J.;Schmaler, Mathias;Landmann, Regine
通讯作者：
Landmann, Regine