Application of a thermo-reversible gelation polymer, mebiol gel, for stem cell culture and regenerative medicine.
Application of a thermo-reversible gelation polymer, mebiol gel, for stem cell culture and regenerative medicine.
复制标题
热可逆聚合物Mebiol凝胶的应用用于干细胞培养和再生医学。
DOI：
10.46582/jsrm.0601003
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发表时间：
2010
影响因子：
2.7
通讯作者：
Huh N
中科院分区：
文献类型：
--
作者：
Kataoka K;Huh N
最近的研究揭示了使用热可逆凝胶聚合物Mebiol Gel的三维培养系统的可能用途。它是一种纯合成的生物相容共聚物，由热响应性聚合物嵌段[聚(N-异丙基丙烯酰胺-甲基丙烯酸丁酯共聚)聚(NIPAAm-co-BMA)]和亲水性聚合物嵌段(聚乙二醇[PEG])组成。Mebiol Gel具有随温度变化的动态粘弹性。Mebiol Gel是一种生物相容性支架，用于三维培养，没有任何毒性。用于三维培养的代表性生物支架，即I型胶原和Matrigel，与细胞相互作用并影响细胞功能，但Mebiol Gel几乎没有这种作用。由于其内源性，Mebiol Gel能够实现单个干细胞的克隆扩增。Mebiol Gel在动物模型组织缺损中的应用表明，Mebiol Gel通过激活干细胞和预防炎症来促进组织再生。因此，Mebiol Gel适合于制备移植细胞，并可直接应用于促进体内受损组织的再生。
Recent studies have revealed the possible utility of a three-dimensional culture system using a thermo-reversible gelation polymer, Mebiol Gel. It is a purely synthesized biocompatible copolymer composed of thermoresponsive polymer blocks [poly(N-isopropylacrylamide-co-n-butyl methacrylate) poly(NIPAAm-co-BMA)] and hydrophilic polymer blocks (polyethylene glycol [PEG]). Mebiol Gel is characterized by its temperature-dependent dynamic visccoelastic properties. Mebiol Gel is used as a biocompatible scaffold for three-dimensional culture without any toxicity. Representative biological scaffolds for three-dimensional culture, i.e. type I collagen and Matrigel, interact with cells and affect cellular functions, but Mebiol Gel hardly showed such effects. Because of its innertness, Mebiol Gel enables clonal expansion of single stem cells. Application of Mebiol Gel to tissue defects in animal models revealed that Mebiol Gel enhanced tissue regeneration with activation of stem cells and prevention of inflammation. Thus, Mebiol Gel is suitable for preparation of cells for transplantation and is useful for direct application to promote regeneration of damaged tissues in vivo.