NATURAL-KILLER-CELL ACTIVITY FROM PREGNANT SUBJECTS IS MODULATED BY RU-486
NATURAL-KILLER-CELL ACTIVITY FROM PREGNANT SUBJECTS IS MODULATED BY RU-486
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DOI：
10.1016/0002-9378(92)91835-x
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发表时间：
1992-01-01
影响因子：
9.8
通讯作者：
KLEIN, TA
中科院分区：
文献类型：
--
作者：
HANSEN, KA;OPSAHL, MS;KLEIN, TA
自然杀伤细胞是人体先天免疫系统的重要组成部分。自然杀伤细胞活动在怀孕期间减少，特别是在后半期。为了研究孕酮在妊娠期免疫调节自然杀伤细胞活性中的作用，我们评估了RU 486对妊娠期分离自然杀伤细胞的影响。以K-562细胞为靶细胞，用18小时铬51释放法测定自然杀伤细胞活性。我们证明，RU 486在5 - 40 μ mol/L的浓度范围内，自然杀伤活性比基线提高了3 - 5倍。这种增强的活动被抑制到基线添加过量的黄体酮。氢化可的松的加入导致这种增强活性的显著降低。本研究提示黄体酮可能在母体接受胎儿同种异体移植中发挥免疫调节因子的作用。
Natural killer cells form an integral component of the body's innate immune system. Natural killer cell activity is reduced during pregnancy, especially in the latter half. To investigate the role progesterone may play in immunomodulating natural killer cell activity during pregnancy, we evaluated the effect of RU 486 on natural killer cells isolated from pregnant subjects. Natural killer cell activity was measured with an 18-hour, Chromium 51 release, microcytotoxicity assay with K-562 cells as target cells. We demonstrated that RU 486, in a concentration range from 5 to 40-mu-mol/L, augmented natural killer activity threefold to fivefold over baseline. This augmentation of activity was suppressed to baseline by the addition of excess progesterone. The addition of hydrocortisone resulted in an insignificant reduction in this augmented activity. This study suggests that progesterone may play a role as an immunomodulating factor in maternal acceptance of the fetal allograft.