Surveillance of Getah virus in mosquitoes and racehorses from 2016 to 2019 at a training center in Ibaraki Prefecture, Japan, a site of several previous Getah virus outbreaks
Surveillance of Getah virus in mosquitoes and racehorses from 2016 to 2019 at a training center in Ibaraki Prefecture, Japan, a site of several previous Getah virus outbreaks
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DOI：
10.1007/s00705-022-05631-3
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发表时间：
2023-01
影响因子：
2.7
通讯作者：
A. Ochi;H. Bannai;Hiroka Aonuma;H. Kanuka;Eri Uchida-Fujii;Yuta Kinoshita;M. Ohta;Yoshinori Kambayashi;K. Tsujimura;T. Ueno;M. Nemoto
中科院分区：
文献类型：
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作者：
A. Ochi;H. Bannai;Hiroka Aonuma;H. Kanuka;Eri Uchida-Fujii;Yuta Kinoshita;M. Ohta;Yoshinori Kambayashi;K. Tsujimura;T. Ueno;M. Nemoto
从2016年到2019年，在米霍训练中心对发热赛马的蚊子和EDTA处理的血液样本进行了Getah病毒感染调查，该中心发生了几次Getah病毒爆发。在本研究中，我们收集了5557只蚊子和331份发热马的血液样本。三带喙库蚊（51.9%）、日本伊蚊（14.2%）和中华按蚊（11.2%）是最常见的蚊种。2016年在蚊子（日本伊蚊）中检测到Getah病毒（菌株16-0810-26），但2017-2019年未检测到。2016年74匹发热马中有6匹和2019年69匹马中有1匹检测出盖塔病毒阳性; 2017年或2018年没有一匹马检测出阳性。系统发育和序列分析表明，菌株16-0810-26与2014-2016年从训练中心周围的马和猪中分离出的菌株密切相关，但自2017年以来在训练中心收集的样品中未检测到。相比之下，2019年从受感染的马中分离出的菌株（19-I-703）与2014-2016年从马和猪中分离出的菌株在遗传上不同，与1978年在训练中心分离出的菌株更密切相关。19-I-703毒株的来源尚不清楚，但在2019年采样的其他马匹中未检测到该病毒。综上所述，我们发现自1979年以来，训练中心的蚊子物种分布没有发生显著变化，尽管2016年训练中心的马中发生了小规模的盖塔病毒感染，但从2017年到2019年，在训练中心的蚊子和马中检测到了有限的盖塔病毒活性。
Mosquitoes and EDTA-treated blood samples from febrile racehorses were investigated for Getah virus infection from 2016 to 2019 at the Miho Training Center, where several outbreaks of Getah virus have occurred. We collected 5557 mosquitoes and 331 blood samples from febrile horses in this study. The most frequently captured mosquito species wasCulex tritaeniorhynchus(51.9%), followed byAedes vexans nipponii(14.2%) andAnopheles sinensis(11.2%). Getah virus was detected in mosquitoes (Aedes vexans nipponii) in 2016 (strain 16-0810-26) but not in 2017-2019. Six of 74 febrile horses in 2016 and one of 69 in 2019 tested positive for Getah virus; none of the horses tested positive in 2017 or 2018. Phylogenetic and sequence analysis showed that strain 16-0810-26 was closely related to strains that had been isolated from horses and a pig around the training center in 2014-2016 but have not been detected in samples collected at the training center since 2017. In contrast, the strain isolated from the infected horse in 2019 (19-I-703) was genetically distinct from the strains isolated from horses and a pig in 2014-2016 and was more closely related to a strain isolated in 1978 at the training center. The source of strain 19-I-703 is unclear, but the virus was not detected in other horses sampled in 2019. In summary, we found that the distribution of mosquito species present at the training center had not changed significantly since 1979, and although a small outbreak of Getah virus infection occurred among horses at the training center in 2016, limited Getah virus activity was detected in mosquitoes and horses at the training center from 2017 to 2019.