Changes in CGRP-immunoreactive nerve fibres during experimental tooth movement in rats.
Changes in CGRP-immunoreactive nerve fibres during experimental tooth movement in rats.
复制标题
大鼠实验性牙齿移动过程中 CGRP 免疫反应性神经纤维的变化。
DOI：
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发表时间：
1990
影响因子：
2.6
通讯作者：
Steinar Kvinnsland
中科院分区：
文献类型：
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作者：
I. Kvinnsland;Steinar Kvinnsland
应用降钙素基因相关肽(CGRP)免疫组织化学方法，观察实验性上颌第一磨牙近中移动过程中牙周和牙髓中表达CGRP神经的变化。正畸矫治器由螺旋弹簧组成，将一侧的上颌第一磨牙连接到中切牙。5d后，持续用力30~50g，超量麻醉后灌流。用抗生物素-生物素-过氧化物酶技术对实验组和对照组颌骨进行连续切片，以显示CGRP免疫反应(IR)神经纤维。诱导的牙齿移动导致CGRP-IR神经的相对数量以及实验牙齿部分神经供应和相关组织结构的形态发生了可重复的变化。大多数实验牙的冠髓和根尖周组织中的CGRP-IR神经数量增加。结果表明，在实验性牙齿移动过程中，牙髓和支持组织中CGRP免疫反应阳性的肽能神经纤维参与了组织反应。
Immunohistochemical localization of calcitonin gene-related peptide (CGRP) was used to investigate changes in nerves expressing CGRP in periodontium and pulp during experimental mesial movement of the first maxillary molar in rats. The orthodontic appliance consisted of a coil spring connecting the first maxillary molar on one side to the central incisors. After 5 days with a continuous force of 30-50 g the animals were perfused after an overdose of anaesthetic. Serial sections from the experimental and control jaws were exposed to the avidin-biotin-peroxidase technique for demonstration of CGRP-immunoreactive (IR) nerve fibres. The induced tooth movement caused reproducible changes in the relative number of CGRP-IR nerves as well as morphological alterations within parts of the nerve supply of the experimental teeth and related tissue structures. The majority of the experimental teeth showed increased number of CGRP-IR nerves in the coronal pulp and periapical tissues. The results indicate that peptidergic nerve fibres immunoreactive for CGRP takes an active part in tissue responses in pulp and supporting tissues during experimental tooth movement.