Investigation of photosynthate-C allocation 27 days after 13C-pulse labeling of Zea mays L. at different growth stages
Investigation of photosynthate-C allocation 27 days after 13C-pulse labeling of Zea mays L. at different growth stages
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DOI：
10.1007/s11104-013-1841-7
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发表时间：
2013-07
期刊：
影响因子：
4.9
通讯作者：
F. Meng;J. Dungait;Xuan-Zhe Zhang;Min He;Yanbin Guo;Wenliang Wu
中科院分区：
文献类型：
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作者：
F. Meng;J. Dungait;Xuan-Zhe Zhang;Min He;Yanbin Guo;Wenliang Wu
目的利用13 C脉冲标记技术对农作物进行示踪研究。然而，很少有研究比较了不同标记时期的C分布。本研究的总体目的是确定13 C脉冲标记后所需的监测间隔长度，以量化玉米整个生长季节光合产物C分配到植物、土壤和根际呼吸池中的情况。方法采用13 CO2（98 at. %）脉冲标记盆栽玉米，分别在玉米出苗初期、拔节期、抽穗期和灌浆期进行标记。13 C脉冲标记后27 d，对13 C进入玉米地上部和根系生物量、土壤CO2通量和土壤有机碳库的途径进行了监测。在出苗期、拔节期、抽穗期和灌浆期，各处理的吸肥率分别为57%、53%、70%和80%。伸长期根系生物量中13 C的回收率（27%）高于灌浆期（3%）。土壤中13 C的回收量在出苗期（2.3%）、拔节期（3.8%）、抽穗期和灌浆期（小于2%）最小。根际呼吸中13 C的回收量，即13 CO2，在出苗时最大（26%），在拔节期，抽穗期和灌浆期相似（~ 16%.ConclusionsAt至少24天，需要有效地监测13 C的恢复后脉冲标记与13 CO2玉米在植物和土壤池。13 C的回收率在不同的生长阶段存在差异，并且与植物代谢需求的变化相对应，表明整个生长季节的标记能够更准确地量化植物-土壤系统中的C收支。
AimsPulse labeling of crops using13C is often employed to trace photosynthesized carbon (C) within crop-soil systems. However, few studies have compared the C distribution for different labeling periods. The overall aim of this study was to determine the length of the monitoring interval required after13C-pulse labeling to quantify photosynthate C allocation into plant, soil and rhizosphere respiration pools for the entire growing season of maize (Zea maysL.).MethodsPot grown maize was pulse-labeled with13CO2(98 at. %) at the beginning of emergence, elongation, heading and grainfilling growth stages. The routing of13C into shoot and root biomass, soil CO2flux and soil organic carbon (SOC) pools was monitored for 27 days after13C-pulse labeling at the beginning of each growth stage.ResultsThe majority of the13C was recovered after 27 d in the maize shoots, i.e., 57 %, 53 %, 70 % and 80 %, at the emergence, elongation, heading, and grainfilling stages, respectively. More13C was recovered in the root biomass at elongation (27 %) compared to the least at the grainfilling stage (3 %). The amount recovered in the soil was the smallest pool of13C at emergence (2.3 %), elongation (3.8 %), heading and grainfilling (less than 2 %). The amount of13C recovered in rhizosphere respiration, i.e.13CO2, was greatest at emergence (26 %), and similar at the elongation, heading and grainfilling stages (~16 %).ConclusionsAt least 24 days is required to effectively monitor the recovery of13C after pulse labeling with13CO2for maize in plant and soil pools. The recovery of13C differed between growth stages and corresponded to the changing metabolic requirements of the plant, which indicated labeling for the entire growth season would more accurately quantify the C budget in plant-soil system.