Expression of kin of irregular chiasm‐like 3/mKirre in proprioceptive neurons of the dorsal root ganglia and its interaction with nephrin in muscle spindles
Expression of kin of irregular chiasm‐like 3/mKirre in proprioceptive neurons of the dorsal root ganglia and its interaction with nephrin in muscle spindles
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DOI：
10.1002/cne.21838
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发表时间：
2008-11
影响因子：
2.5
通讯作者：
T. Komori;Hiroaki Gyobu;Hiroo Ueno;T. Kitamura;E. Senba;Y. Morikawa
中科院分区：
文献类型：
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作者：
T. Komori;Hiroaki Gyobu;Hiroo Ueno;T. Kitamura;E. Senba;Y. Morikawa
Kin of irregular chiasm‐like 3（Kirrel 3）是果蝇kirre基因的哺乳动物同源物，属于免疫球蛋白超家族。此前，我们已经报道了Kirrel 3在发育和成人中枢神经系统中表达。本研究探讨Kirrel 3在小鼠背根神经节（DRG）及其投射靶区的表达。在成年DRG中，Kirrel 3 mRNA在总DRG神经元的21.5 ± 2.3%中检测到，并且主要在中型和大型神经元中表达。此外，Kirrel 3 mRNA主要与酪氨酸激酶受体（Trk）C免疫反应性共定位。在发育中的DRG中，Kirrel 3 mRNA在胚胎第11.5天在少数细胞中首次检测到，随后逐渐增加，并在E17.5时达到成体水平。在发育过程中，Kirrel 3在大多数TrkC阳性DRG神经元中表达。在E17.5时，在肌梭的神经营养因子3（NT 3）阳性梭内肌纤维周围的TrkC阳性神经纤维中观察到Kirrel 3的表达。然而，在整个发育过程中，Kirrel 3在投射到脊髓的TrkC阳性神经纤维中不表达。此外，nephrin在NT 3阳性梭内肌纤维中表达，并与Kirrel 3免疫反应性密切相关。免疫共沉淀实验表明nephrin与Kirrel 3在肌肉发育过程中相互作用。这些结果表明，Kirrel 3可能在轴突寻路、细胞识别和DRG神经元在适当靶细胞上的突触形成中发挥作用，包括通过与nephrin相互作用靶向肌梭上的本体感受神经元。J. Comp.神经元511：92-108，2008.© 2008 Wiley利斯公司
Kin of irregular chiasm‐like 3 (Kirrel3), a mammalian homolog of the kirre gene of Drosophila melanogaster, belongs to the immunoglobulin superfamily. Previously, we have reported that Kirrel3 is expressed in the developing and adult central nervous system. In the present study we investigated the expression of Kirrel3 in the mouse dorsal root ganglia (DRG) and their projection targets. In the adult DRGs, Kirrel3 mRNA was detected in 21.5 ± 2.3% of total DRG neurons and the expression was mainly prevalent in the medium‐ and large‐sized neurons. In addition, Kirrel3 mRNA predominantly colocalized with tyrosine kinase receptor (Trk) C‐immunoreactivity. In the developing DRGs, Kirrel3 mRNA was first detected in a few cells at embryonic day (E) 11.5, gradually increased, and reached the adult level at E17.5. During the development, Kirrel3 was expressed in most TrkC‐positive DRG neurons. The expression of Kirrel3 was observed in TrkC‐positive nerve fibers around neurotrophin 3 (NT3)‐positive intrafusal muscle fibers of muscle spindles at E17.5. However, Kirrel3 was not expressed in TrkC‐positive nerve fibers projecting to the spinal cord throughout development. Furthermore, nephrin was expressed in the NT3‐positive intrafusal muscle fibers and was in close apposition with Kirrel3‐immunoreactivity. Coimmunoprecipitation assay revealed that nephrin interacted with Kirrel3 in the developing muscles. These results suggest that Kirrel3 might play a role in the axonal pathfinding, cell recognition, and synapse formation of DRG neurons on appropriate target cells, including the targeting of proprioceptive neurons on muscle spindles through the interaction with nephrin. J. Comp. Neurol. 511:92–108, 2008. © 2008 Wiley‐Liss, Inc.