Using Evans Blue Dye to Determine Blood-Brain Barrier Integrity in Rodents.
Using Evans Blue Dye to Determine Blood-Brain Barrier Integrity in Rodents.
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DOI：
10.1002/cpim.83
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发表时间：
2019-09-01
影响因子：
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通讯作者：
Petronilho, Fabricia
中科院分区：
文献类型：
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作者：
Goldim, Mariana Pereira de Souza;Della Giustina, Amanda;Petronilho, Fabricia
血脑屏障（blood-brain barrier，BBB）是一种主动的、选择性的屏障，它保护脑免受内源性和外源性的损伤。不同的刺激可能导致这种屏障的破坏，包括炎症和创伤。几种方法用于评估BBB破坏。最广泛使用的方法是伊文思蓝（EB）染料外渗。EB通常不能通过BBB，因此其在脑组织中的存在表明渗透性的改变。本方案详细描述了啮齿动物中EB外渗的步骤。还提供了关于关键步骤和优点的重要方面。John Wiley & Sons，Inc._关于我们
The blood-brain barrier (BBB) is an active and selective barrier that shields the brain from endogenous and exogenous insults. Different stimuli may lead to the disruption of this barrier, including inflammation and trauma. Several methods are used to evaluate BBB disruption. The most widely used method is Evans blue (EB) dye extravasation. EB cannot normally pass through the BBB and thus its presence in brain tissue indicates alterations in permeability. This protocol details the steps of EB extravasation in rodents. Important aspects regarding critical steps and advantages are also provided. © 2019 by John Wiley & Sons, Inc.