Oxidative stress is an early symptom triggered by aluminum in Al-sensitive potato plantlets.
Oxidative stress is an early symptom triggered by aluminum in Al-sensitive potato plantlets.
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DOI：
10.1016/j.chemosphere.2009.06.011
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发表时间：
2009-09
期刊：
影响因子：
8.8
通讯作者：
L. A. Tabaldi;D. Cargnelutti;J. Gonçalves;L. B. Pereira;G. Y. Castro;J. Maldaner;R. Rauber;L. Rossato;D. Bisognin;M. Schetinger;F. Nicoloso
中科院分区：
文献类型：
--
作者：
L. A. Tabaldi;D. Cargnelutti;J. Gonçalves;L. B. Pereira;G. Y. Castro;J. Maldaner;R. Rauber;L. Rossato;D. Bisognin;M. Schetinger;F. Nicoloso
本研究的目的是评估是否铝（Al）毒性引起的氧化应激是一个早期症状，可以触发根生长抑制猕猴（铝敏感）和SMIC 148-A（铝耐受）马铃薯克隆。将小植株生长在含有0、100和200 mg Al L-1的营养液（pH 4.00）中。铝处理后24、72、120和168 h，测定根长和生化指标。无论暴露时间如何，猕猴克隆的根长度在200 mg Al L−1时显着降低。对于SMIC 148-A无性系，根长没有随着任何Al处理而降低。供铝仅引起猕猴根系（24、72、120和168 h）和地上部（120和168 h）的脂质过氧化。在猕猴根中，过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性分别在72和120 h，24，72和120 h下降。在SMIC 148-A的根中，CAT活性在72和168 h时升高，APX活性在72 h时下降，在24、12和168 h时升高。在所有评价中，猕猴在200 mg Al L− 1下显示出较低的根部非蛋白巯基（NPSH）浓度，但SMIC 148-A在24和72 h时未显示出任何变化，或在120 h时呈现出较高的水平。在24和120 h的根抗坏血酸（阿萨）浓度中也观察到这种模式。这些马铃薯克隆的细胞氧化还原状态似乎受到铝的影响。因此，氧化应激可能是铝毒性的重要机制，主要是在铝敏感的猕猴克隆。
The objective of this study was to evaluate whether the oxidative stress caused by aluminum (Al) toxicity is an early symptom that can trigger root growth inhibition in Macaca (Al-sensitive) and SMIC148-A (Al-tolerant) potato clones. Plantlets were grown in a nutrient solution (pH 4.00) with 0, 100 and 200mg Al L−1. At 24, 72, 120 and 168h after Al addition, root length and biochemical parameters were determined. Regardless of exposure time, root length of the Macaca clone was significantly lower at 200mg Al L−1. For the SMIC148-A clone, root length did not decrease with any Al treatments. Al supply caused lipid peroxidation only in Macaca, in both roots (at 24, 72, 120 and 168h) and shoot (at 120 and 168h). In roots of the Macaca, catalase (CAT) and ascorbate peroxidase (APX) activity decreased at 72 and 120h, and at 24, 72 and 120h, respectively. At 168h, both activities increased upon addition of Al. In roots of the SMIC148-A, CAT activity increased at 72 and 168h, whereas APX activity decreased at 72h and increased at 24, 12 and 168h. The Macaca showed lower root non-protein thiol group (NPSH) concentration at 200mg Al L−1in all evaluations, but the SMIC148-A either did not demonstrate any alterations at 24 and 72h or presented higher levels at 120h. This pattern was also observed in root ascorbic acid (AsA) concentration at 24 and 120h. The cellular redox status of these potato clones seems to be affected by Al. Therefore, oxidative stress may be an important mechanism for Al toxicity, mainly in the Al-sensitive Macaca clone.