Experimental and simulation study of the wavelength dependent second harmonic generation of collagen in scattering tissues.
Experimental and simulation study of the wavelength dependent second harmonic generation of collagen in scattering tissues.
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DOI：
10.1364/ol.39.001897
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发表时间：
2014-04-01
期刊：
影响因子：
3.6
通讯作者：
Campagnola PJ
中科院分区：
文献类型：
--
作者：
Hall G;Tilbury KB;Campbell KR;Eliceiri KW;Campagnola PJ
我们报道了在800-1200 nm的波长范围内散射组织中胶原蛋白的二次谐波产生(SHG)的波长依赖性。这项研究包含了胶原蛋白的分子超极化率β和光学散射，这两者都是波长相关的。利用三维倍频成像和蒙特卡罗模拟，我们发现基于已知吸收带的二态模型不能很好地描述β的波长依赖关系。我们进一步发现，较长波长的激发是无效的，因为散射的减少被远离共振的β的减小所抵消，并且最佳激发在800-900 nm范围内。对于反向收集的倍频，影响更大。
We report on the wavelength dependence of second harmonic generation (SHG) of collagen in scattering tissues over the wavelength range of 800–1200 nm. The study incorporates inclusion of the molecular hyperpolarizability β of collagen and optical scattering, both of which are wavelength dependent. Using 3D SHG imaging and Monte Carlo simulations, we find the wavelength dependence of β is not well described by a two-state model based on known absorption bands. We further find that longer wavelength excitation is inefficient as the reduction in scattering is overcome by the decreased β far from resonance and the optimal excitation is within the 800–900 nm range. The impact is larger for backward collected SHG.